{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:46:40Z","timestamp":1792028800860,"version":"4.4.0"},"reference-count":13,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:00:00Z","timestamp":1791936000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007451","name":"Cell Science Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007451","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008886","name":"Princess Takamatsu Cancer Research Fund","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008886","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K27123"],"award-info":[{"award-number":["23K27123"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K02404"],"award-info":[{"award-number":["25K02404"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26K23403"],"award-info":[{"award-number":["26K23403"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["26K01956"],"award-info":[{"award-number":["26K01956"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["24K22065"],"award-info":[{"award-number":["24K22065"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007684","name":"Asahi Glass Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007684","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Traffic"],"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Recent work by Hirata and colleagues identifies site\u2010specific\n                    <jats:italic>O<\/jats:italic>\n                    \u2010GlcNAc modification of the cargo\u2010selecting COPII subunit Sec24D as a dynamic regulatory switch that promotes collagen export from the endoplasmic reticulum (ER), providing new insight into how COPII accommodates cargoes far larger than canonical transport vesicles. Two glycosites in the poorly characterized N\u2010terminal intrinsically disordered region (IDR) of Sec24D, T9 and S13, display strikingly different dynamics: S13 is constitutively glycosylated, whereas T9 is rapidly modified following a synchronized burst of collagen transport and returns to baseline within minutes. Both sites are required for collagen I trafficking and rescue of Sec24D\u2010deficient\n                    <jats:italic>bulldog<\/jats:italic>\n                    zebrafish, while T9 is also required for efficient transport of the smaller cargo E\u2010cadherin, suggesting that a single intrinsically disordered region can encode cargo\u2010selective information. Crosslinking proteomics further reveals distinct glycosylation\u2010dependent changes in the Sec24D interactome and identifies myoferlin (MYOF) as a partner proposed to promote fusion of ER exit sites (ERES) with ER\u2010Golgi intermediate compartment (ERGIC) membranes. In this commentary, we discuss how these findings recast the COPII inner coat as a signal\u2010integrating platform, intersect with TANGO1\u2010dependent ERGIC recruitment and competing models of large collagen carriers, and raise key mechanistic questions concerning signal transmission across the ER membrane, glycan stoichiometry, and how the recruitment or tethering function proposed for MYOF is coupled to membrane fusion.\n                  <\/jats:p>","DOI":"10.1111\/tra.70055","type":"journal-article","created":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:36:01Z","timestamp":1792028161000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sugar on the Coat: Site\u2010Specific O\u2010\n                    <scp>GlcNAc<\/scp>\n                    and Myoferlin Adapt\n                    <scp>COPII<\/scp>\n                    for Collagen Export"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5573-2167","authenticated-orcid":false,"given":"Miharu","family":"Maeda","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Graduate School of Medicine Akita University  Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2478-2687","authenticated-orcid":false,"given":"Kota","family":"Saito","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Graduate School of Medicine Akita University  Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.ajhg.2015.01.002","article-title":"Mutations in Sec24D, Encoding a Component of the COPII Machinery, Cause a Syndromic Form of Osteogenesis Imperfecta","volume":"96","author":"Garbes L.","year":"2015","journal-title":"American Journal of Human Genetics"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0010367","article-title":"Sec24D\u2010Dependent Transport of Extracellular Matrix Proteins Is Required for Zebrafish Skeletal Morphogenesis","volume":"5","author":"Sarmah S.","year":"2010","journal-title":"PLoS One"},{"issue":"1","key":"e_1_2_5_4_1","doi-asserted-by":"crossref","first-page":"6415","DOI":"10.1038\/s41467-026-73027-x","article-title":"Site\u2010Specific Glycosylation of Sec24D and Myoferlin Recruit ERGIC to ER Exit Sites for Collagen Trafficking","volume":"17","author":"Hirata T.","year":"2026","journal-title":"Nature Communications"},{"issue":"1","key":"e_1_2_5_5_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1021\/acs.biochem.7b00870","article-title":"Dynamic Glycosylation Governs the Vertebrate COPII Protein Trafficking Pathway","volume":"57","author":"Cox N. J.","year":"2018","journal-title":"Biochemistry"},{"issue":"24","key":"e_1_2_5_6_1","doi-asserted-by":"crossref","first-page":"5565","DOI":"10.1242\/dev.02155","article-title":"Normal Myoblast Fusion Requires Myoferlin","volume":"132","author":"Doherty K. R.","year":"2005","journal-title":"Development"},{"key":"e_1_2_5_7_1","doi-asserted-by":"crossref","DOI":"10.7554\/eLife.10982","article-title":"TANGO1 Recruits ERGIC Membranes to the Endoplasmic Reticulum for Procollagen Export","volume":"4","author":"Santos A. 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V.","year":"2021","journal-title":"Cell"},{"issue":"8","key":"e_1_2_5_11_1","doi-asserted-by":"crossref","DOI":"10.1083\/jcb.202603129","article-title":"Procollagen 1 Assembles Into Phase\u2010Separated Condensates in the Endoplasmic Reticulum","volume":"225","author":"Bhattacharyya S.","year":"2026","journal-title":"Journal of Cell Biology"},{"issue":"12","key":"e_1_2_5_12_1","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1038\/s41580-025-00899-0","article-title":"Towards a Unified Framework for the Function of Endoplasmic Reticulum Exit Sites","volume":"26","author":"Farhan H.","year":"2025","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1038\/nature10822","article-title":"Ubiquitin\u2010Dependent Regulation of COPII Coat Size and Function","volume":"482","author":"Jin L.","year":"2012","journal-title":"Nature"},{"issue":"1","key":"e_1_2_5_14_1","doi-asserted-by":"crossref","first-page":"10434","DOI":"10.1038\/s41467-025-65409-4","article-title":"Phosphorylation\u2010Coupled Autoregulation of TANGO1 and Sec16A Maintains Functional ER Exit Sites","volume":"16","author":"Maeda M.","year":"2025","journal-title":"Nature Communications"}],"container-title":["Traffic"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.70055","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/tra.70055","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/tra.70055","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T01:36:04Z","timestamp":1792028164000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/tra.70055"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,14]]},"references-count":13,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2026,12]]}},"alternative-id":["10.1111\/tra.70055"],"URL":"https:\/\/doi.org\/10.1111\/tra.70055","archive":["Portico"],"relation":{},"ISSN":["1398-9219","1600-0854"],"issn-type":[{"value":"1398-9219","type":"print"},{"value":"1600-0854","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,14]]},"assertion":[{"value":"2026-08-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-06","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-10-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70055"}}