{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T13:26:24Z","timestamp":1779888384323,"version":"3.53.1"},"reference-count":11,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T00:00:00Z","timestamp":1359504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Human herpesvirus 8 (<jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108) is likely transmitted through blood transfusion in high\u2010prevalence areas. The efficacy of leukoreduction filtration for reducing <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 in blood has not been reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Blood was drawn from 45 human immunodeficiency virus\u2013positive men either with Kaposi's sarcoma (<jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content>; n\u2009=\u200921) or without <jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content> (n\u2009=\u200924) and subject to leukoreduction filtration. <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 viral load was measured in plasma and in blood before and after filtration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Twelve subjects, all with <jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content>, had detectable <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 viremia before filtration with viral loads of 10<jats:sup>2<\/jats:sup> to 10<jats:sup>5<\/jats:sup> copies\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> (mean, 3\u2009\u00d7\u200910<jats:sup>4<\/jats:sup> copies\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content>). After filtration, seven of 12 subjects no longer had detectable <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 in their blood, and five of 12 subjects had detectable <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 that was 90% reduced on average from prefiltration levels. The presence of <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 in the blood after filtration was strongly associated with prefiltration viral loads greater than 1000 copies\/<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> and the presence of cell\u2010free virus in plasma. None of the subjects without <jats:styled-content style=\"fixed-case\">KS<\/jats:styled-content> had detectable levels of <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 virus in blood before or after filtration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Cell\u2010associated <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 appeared to be effectively removed by leukoreduction filtration. Cell\u2010free <jats:styled-content style=\"fixed-case\">HHV<\/jats:styled-content>\u20108 was present in 42% of subjects as 1% to 20% of the total virus which was not removed by filtration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12108","type":"journal-article","created":{"date-parts":[[2013,1,30]],"date-time":"2013-01-30T11:53:44Z","timestamp":1359546824000},"page":"2164-2167","source":"Crossref","is-referenced-by-count":16,"title":["Measurements of human herpesvirus 8 viral load in blood before and after leukoreduction filtration"],"prefix":"10.1111","volume":"53","author":[{"given":"Sheila C.","family":"Dollard","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia"},{"name":"HIV\/AIDS Clinical Trials Unit Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Roback","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia"},{"name":"HIV\/AIDS Clinical Trials Unit Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford","family":"Gunthel","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia"},{"name":"HIV\/AIDS Clinical Trials Unit Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minal M.","family":"Amin","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia"},{"name":"HIV\/AIDS Clinical Trials Unit Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheilagh","family":"Barclay","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia"},{"name":"HIV\/AIDS Clinical Trials Unit Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ericka","family":"Patrick","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia"},{"name":"HIV\/AIDS Clinical Trials Unit Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Kuehnert","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention  Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine Emory University School of Medicine  Atlanta Georgia"},{"name":"HIV\/AIDS Clinical Trials Unit Emory University School of Medicine  Atlanta 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