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Processing <jats:styled-content style=\"fixed-case\">UCB<\/jats:styled-content> by <jats:styled-content style=\"fixed-case\">HESPAN<\/jats:styled-content>\u20106% and anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34\u2010<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>iltenyi particles provides insufficient cells for treating adults. Physicochemical\u2010electrokinetic studies on <jats:styled-content style=\"fixed-case\">UCB<\/jats:styled-content>\u2010mononuclear cells (MNCs) under conditions of delayed processing, ice or very low temperatures, and some cell separation media identified artifacts introduced by procedures. Adsorption of biomaterials from cell damage by temperature, degradation products after using enzymes, harsh reagents, dithiothreitol, and <jats:styled-content style=\"fixed-case\">HESPAN<\/jats:styled-content> affect cell properties and distribution. Miltenyi particles internalized by cells could release iron that accumulating in liver or spleen would then risk toxicity. Summary topics included the effects of temperature, <jats:styled-content style=\"fixed-case\">HESPAN<\/jats:styled-content> (fast sedimenting agent), glycoproteases, <jats:styled-content style=\"fixed-case\">DNase<\/jats:styled-content>, and dithiothreitol risk affecting cell receptors in recognition, \u201choming,\u201d leading to possible unintended iatrogenic bioeffects should such cells be transfused into humans. The loss of undetectable and uncaptured low <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 antigen\u2013bearing cells by <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>iltenyi particles seems to occur when the current methods of isolation of CD34+ cells and other cells are critically assessed. The purpose here is to highlight and suggest avoiding the procedural flaws involved. Preventing ice temperatures avoids ice\u2010damaged platelets releasing biomaterials that are adsorbed on cells altering <jats:styled-content style=\"fixed-case\">UBC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content>s\/<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> properties and cell loss. Omitting the positive selection with antibody\u2010linked Miltenyi particles obviates the use of harsh reagents to release the cells. <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>nternalized <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>iltenyi particles are a toxicity hazard that needs investigations. Achieving approximately 5% yields of CD34+ cells (153\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>\/110\u2009<jats:styled-content style=\"fixed-case\">mL<\/jats:styled-content> cord\u2010placenta blood) is a major advance holding great promise, for the first time increasing the prospect of stem cell therapy of 70\u2010kg adults, using a single <jats:styled-content style=\"fixed-case\">UCB<\/jats:styled-content> donation (with dose of 1.5\u2009\u00d7\u200910<jats:sup>5<\/jats:sup> cells\/kg) and considerably cheaper cultured red blood cells manufacture (multiple packs\/2\u2009\u00d7\u200910<jats:sup>12<\/jats:sup>).<\/jats:p>","DOI":"10.1111\/trf.12122","type":"journal-article","created":{"date-parts":[[2013,2,22]],"date-time":"2013-02-22T16:01:07Z","timestamp":1361548867000},"page":"2667-2674","source":"Crossref","is-referenced-by-count":4,"title":["Current and historical perspectives on methodological flaws in processing umbilical cord blood"],"prefix":"10.1111","volume":"53","author":[{"given":"J.N.","family":"Mehrishi","sequence":"first","affiliation":[{"name":"University of Cambridge  Cambridge UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,2,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198910263211707"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199708073370602"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2009.005694"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3828"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2184.2010.00728.x"},{"key":"e_1_2_8_7_1","first-page":"1","volume-title":"Progress in biophysics and molecular biology","author":"Mehrishi JN","year":"1972"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1979.tb01375.x"},{"key":"e_1_2_8_9_1","first-page":"411","volume-title":"Cell electrophoresis 84","author":"Mehrishi JN","year":"1985"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0090-1229(85)90096-0"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02989692"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12123"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI111645"},{"key":"e_1_2_8_13_2","first-page":"818","volume-title":"Leukocyte typing IV. 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