{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T11:31:04Z","timestamp":1770895864290,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,2,27]],"date-time":"2013-02-27T00:00:00Z","timestamp":1361923200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2013,11]]},"abstract":"Background<\/jats:title>B<\/jats:styled-content>abesia microti<\/jats:italic>, an intraerythrocytic parasite, has been implicated in transfusion transmission. B<\/jats:styled-content>.\u2009microti<\/jats:italic> seroprevalence in Connecticut (CT<\/jats:styled-content>) blood donors is approximately 1%; however, it is not known what percentage of donors is parasitemic and poses a risk for transmitting infection. Therefore, we determined the prevalence of demonstrable B<\/jats:styled-content>.\u2009microti<\/jats:italic>\u2005DNA<\/jats:styled-content> in donors from a highly endemic area of CT<\/jats:styled-content> and compared observed rates with concurrent immunofluorescence assay (IFA<\/jats:styled-content>) testing results.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>Blood samples from consenting donors in southeastern CT<\/jats:styled-content> were collected from mid\u2010A<\/jats:styled-content>ugust through early O<\/jats:styled-content>ctober 2009 and tested by IFA<\/jats:styled-content> for immunoglobulin G<\/jats:styled-content> antibodies and real\u2010time polymerase chain reaction (PCR<\/jats:styled-content>) for B<\/jats:styled-content>.\u2009microti<\/jats:italic>\u2005DNA<\/jats:styled-content>. IFA<\/jats:styled-content> specificity was determined using blood donor samples collected in northwestern Vermont (VT<\/jats:styled-content>), an area nonendemic for B<\/jats:styled-content>abesia<\/jats:italic>.<\/jats:p><\/jats:sec>Results<\/jats:title>Of 1002 CT<\/jats:styled-content> donors, 25 (2.5%) were IFA<\/jats:styled-content> positive and three (0.3%) were real\u2010time PCR<\/jats:styled-content> positive. Among the three real\u2010time PCR<\/jats:styled-content>\u2013positive donors, two were also IFA<\/jats:styled-content> positive, while one was IFA<\/jats:styled-content> negative and may represent a window period infection. The two IFA<\/jats:styled-content>\u2010 and real\u2010time PCR<\/jats:styled-content>\u2013positive donors appeared to subsequently clear infection. The other real\u2010time PCR<\/jats:styled-content>\u2013positive donor did not provide follow\u2010up samples. Of 1015 VT<\/jats:styled-content> donors tested by IFA<\/jats:styled-content>, only one (0.1%) was positive, but may have acquired infection during travel to an endemic area.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>We prospectively identified several real\u2010time PCR<\/jats:styled-content>\u2013positive blood donors, including an IFA<\/jats:styled-content>\u2010negative real\u2010time PCR<\/jats:styled-content>\u2013positive donor, in an area highly endemic for B<\/jats:styled-content>.\u2009microti<\/jats:italic>. These results suggest the need to include nucleic acid testing in planned mitigation strategies for B<\/jats:styled-content>.\u2009microti<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12125","type":"journal-article","created":{"date-parts":[[2013,2,28]],"date-time":"2013-02-28T22:25:53Z","timestamp":1362090353000},"page":"2644-2649","source":"Crossref","is-referenced-by-count":25,"title":["B<\/scp>abesia microti<\/i> real\u2010time polymerase chain reaction testing of C<\/scp>onnecticut blood donors: potential implications for screening algorithms"],"prefix":"10.1111","volume":"53","author":[{"given":"Stephanie T.","family":"Johnson","sequence":"first","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Farmington Connecticut"},{"name":"Biomedical Services Research Department, Northeast Division American Red Cross Farmington Connecticut"},{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Rockville Maryland"},{"name":"Research Division Imugen Norwood Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric R.","family":"Van Tassell","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Farmington Connecticut"},{"name":"Biomedical Services Research Department, Northeast Division American Red Cross Farmington Connecticut"},{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Rockville Maryland"},{"name":"Research Division Imugen Norwood Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Tonnetti","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Farmington Connecticut"},{"name":"Biomedical Services Research Department, Northeast Division American Red Cross Farmington Connecticut"},{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Rockville Maryland"},{"name":"Research Division Imugen Norwood Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ritchard G.","family":"Cable","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Farmington Connecticut"},{"name":"Biomedical Services Research Department, Northeast Division American Red Cross Farmington Connecticut"},{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Rockville Maryland"},{"name":"Research Division Imugen Norwood Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor P.","family":"Berardi","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Farmington Connecticut"},{"name":"Biomedical Services Research Department, Northeast Division American Red Cross Farmington Connecticut"},{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Rockville Maryland"},{"name":"Research Division Imugen Norwood Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Leiby","sequence":"additional","affiliation":[{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Farmington Connecticut"},{"name":"Biomedical Services Research Department, Northeast Division American Red Cross Farmington Connecticut"},{"name":"Transmissible Diseases Department American Red Cross Holland Laboratory Rockville Maryland"},{"name":"Research Division Imugen Norwood Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,2,27]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1202018"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00022-10"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-155-8-201110180-00362"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02317.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02380.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03345.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00609.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02430.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/169.4.923"},{"key":"e_1_2_8_11_1","unstructured":"Vermont Department of Health.Babesiosis.2012. 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