{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T18:26:57Z","timestamp":1776450417303,"version":"3.51.2"},"reference-count":59,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2013,3,5]],"date-time":"2013-03-05T00:00:00Z","timestamp":1362441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2013,12]]},"abstract":"Background<\/jats:title>Lysophosphatidylcholine (LPC<\/jats:styled-content>) has been implicated in the onset of transfusion\u2010related acute lung injury (TRALI<\/jats:styled-content>). In plasma, LPC<\/jats:styled-content> is converted to lysophosphatidic acid (LPA<\/jats:styled-content>) by autotaxin (ATX<\/jats:styled-content>). The effect of leukoreduction in the accumulation of these bioactive lipids and ATX<\/jats:styled-content> in human autologous blood has not been fully investigated.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>The accumulation of choline\u2010containing phospholipids (LPC<\/jats:styled-content>, sphingomyelin [SM<\/jats:styled-content>], and phosphatidylcholine [PC<\/jats:styled-content>]), LPA<\/jats:styled-content>, and ATX<\/jats:styled-content> during the storage of autologous blood and the changes caused by leukoreduction were investigated. A total of 26 orthopedic patients were enrolled. Autologous blood was collected as whole blood and, after leukoreduction, preserved refrigerated until use. Prestorage leukoreduced (LR<\/jats:styled-content>) and non\u2010LR<\/jats:styled-content> autologous blood samples were analyzed. The time\u2010dependent changes and the effect of the filtration were compared.<\/jats:p><\/jats:sec>Results<\/jats:title>A time\u2010dependent and significant increase in the levels of LPA<\/jats:styled-content> was observed in both non\u2010LR<\/jats:styled-content> and LR<\/jats:styled-content> samples. The concentration of LPA<\/jats:styled-content> was significantly reduced in LR<\/jats:styled-content> compared to non\u2010LR<\/jats:styled-content> samples. The concentration of LPC<\/jats:styled-content> was higher in LR<\/jats:styled-content> compared to non\u2010LR<\/jats:styled-content> samples. The levels of PC<\/jats:styled-content>, SM<\/jats:styled-content>, and ATX<\/jats:styled-content> were not affected by either the storage period or the leukoreduction.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Leukoreduction of autologous whole blood effectively reduced the accumulation of LPA<\/jats:styled-content>. On the other hand, prestorage leukoreduction resulted in an increased concentration of LPC<\/jats:styled-content>, without significantly affecting ATX<\/jats:styled-content>. Further studies are necessary to confirm the role of LPA<\/jats:styled-content> in the pathogenesis of adverse effects of blood transfusion, especially TRALI<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12152","type":"journal-article","created":{"date-parts":[[2013,3,6]],"date-time":"2013-03-06T05:06:46Z","timestamp":1362546406000},"page":"3139-3148","source":"Crossref","is-referenced-by-count":3,"title":["Inhibition of lysophosphatidic acid increase by prestorage whole blood leukoreduction in autologous CPDA<\/scp>\u20101 whole blood"],"prefix":"10.1111","volume":"53","author":[{"given":"Yutaka","family":"Nagura","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine University of Tokyo Hospital Tokyo Japan"},{"name":"Department of Clinical Laboratory University of Tokyo Hospital Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelson H.","family":"Tsuno","sequence":"additional","affiliation":[{"name":"Department 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