{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T12:50:20Z","timestamp":1753275020641},"reference-count":24,"publisher":"Wiley","issue":"10pt2","license":[{"start":{"date-parts":[[2013,10,17]],"date-time":"2013-10-17T00:00:00Z","timestamp":1381968000000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2013,10]]},"abstract":"Background<\/jats:title>The risk of transferring blood\u2010borne infections during transfusion is continually increasing because of newly emerging and reemerging viruses. Development of a rapid screening method for emerging viruses that might be transmitted by transfusion is required to eliminate such pathogens during blood donor screening. Owing to increased use of human materials in organ transplants and cell therapy, the risk of donor\u2010transmitted viral infections is also increasing. Although nucleic acid amplification technology (NAT<\/jats:styled-content>) is dedicated to blood screening, a small, convenient detection system is needed at the laboratory and hospital level.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>We developed a new pathogen detection system that can detect multiple viruses simultaneously, using originally designed degenerate polymerase chain reaction primers to amplify a wide range of viral genotypes. Amplified samples were identified using a DNA<\/jats:styled-content> microarray of pathogen\u2010specific probes.<\/jats:p><\/jats:sec>Results<\/jats:title>We detected very low copy numbers of multiple subtypes of viruses, such as human hepatitis C<\/jats:styled-content> virus (HCV<\/jats:styled-content>), human hepatitis B<\/jats:styled-content> virus (HBV<\/jats:styled-content>), human parvovirus B<\/jats:styled-content>19 (PVB<\/jats:styled-content>19), and West Nile virus (WNV<\/jats:styled-content>), using a single plate. We also detected all genotypes of human immunodeficiency virus (HIV<\/jats:styled-content>) but sensitivity was less than for the other viruses.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>We developed a microarray assay using novel primers for detection of a wide range of multiple pathogens and subtypes. Our NAT<\/jats:styled-content> system was accurate and reliable for detection of HIV<\/jats:styled-content>, HBV<\/jats:styled-content>, HCV<\/jats:styled-content>, PVB<\/jats:styled-content>19, and WNV<\/jats:styled-content>, with respect to specificity, sensitivity, and genotype inclusivity. Our system could be customized and extended for emerging pathogens and is suitable as a future NAT<\/jats:styled-content> system.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12193","type":"journal-article","created":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T10:51:25Z","timestamp":1366195885000},"page":"2545-2555","source":"Crossref","is-referenced-by-count":3,"title":["Degenerate polymerase chain reaction strategy with DNA<\/scp> microarray for detection of multiple and various subtypes of virus during blood screening"],"prefix":"10.1111","volume":"53","author":[{"given":"Kazuya","family":"Takizawa","sequence":"first","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tatsuo","family":"Nakashima","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takuo","family":"Mizukami","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madoka","family":"Kuramitsu","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daiji","family":"Endoh","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeto","family":"Kawauchi","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kohsuke","family":"Sasaki","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haruka","family":"Momose","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiharu","family":"Kiba","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Mizutani","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rika A.","family":"Furuta","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazunari","family":"Yamaguchi","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isao","family":"Hamaguchi","sequence":"additional","affiliation":[{"name":"Department of Safety Research on Blood and Biologics Department of Virology, Medicine National Institute of Infectious Diseases Tokyo Japan"},{"name":"Department of Pathology Yamaguchi University Graduate School of Medicine Yamaguchi Japan"},{"name":"Department of Veterinary Medicine Rakuno Gakuen University Hokkaido Japan"},{"name":"Osaka Red Cross Blood Center Osaka Japan"},{"name":"Nihon Parkerizing Hiroshima Works Co. Ltd Hiroshima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,10,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00183.x"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02279.x"},{"key":"e_1_2_8_4_1","first-page":"102","article-title":"Quality assessment of hepatitis C virus nucleic acid amplification methods","volume":"25","author":"Lelie PN","year":"1998","journal-title":"Infus Ther Transfus Med"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e31818b6548"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.10.045"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000080872"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0042-9007.2004.00406.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa030969"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1403.070463"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2004.01.017"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.21309"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-008-9173-8"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr070083j"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-7-91"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.10.e43"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12033-007-0039-8"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90123-6"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705-007-1053-7"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1053\/jhep.2001.27221"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1086\/382885"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79811-0"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02156.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02081-08"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.03.087"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.12193","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.12193","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T11:29:32Z","timestamp":1694604572000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.12193"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,10]]},"references-count":24,"journal-issue":{"issue":"10pt2","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1111\/trf.12193"],"URL":"https:\/\/doi.org\/10.1111\/trf.12193","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,10]]}}}