{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,2]],"date-time":"2024-06-02T02:31:25Z","timestamp":1717295485436},"reference-count":43,"publisher":"Wiley","issue":"11pt2","license":[{"start":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T00:00:00Z","timestamp":1365379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2013,11]]},"abstract":"Background<\/jats:title>The numerous antigens in the K<\/jats:styled-content>ell blood group system result from missense nucleotide changes in KEL<\/jats:italic><\/jats:styled-content>. Antibodies to antigens in this system can be clinically important. We describe six probands whose plasma contained antibodies to high\u2010prevalence K<\/jats:styled-content>ell antigens and discuss their relationship.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>Polymerase chain reaction amplification, direct sequencing, restriction fragment length polymorphism assays, hemagglutination, flow cytometry, and protein modeling were performed by standard methods.<\/jats:p><\/jats:sec>Results<\/jats:title>P<\/jats:styled-content>roband 1 (KUCI<\/jats:styled-content>) and her serologically compatible sister were heterozygous for a nucleotide change in Exon 11 (KEL<\/jats:italic><\/jats:styled-content>*<\/jats:italic>1271C<\/jats:styled-content>\/T<\/jats:styled-content>; A<\/jats:styled-content>la424V<\/jats:styled-content>al). Proband 2 (KANT<\/jats:styled-content>) was heterozygous for KEL<\/jats:italic><\/jats:styled-content>*<\/jats:italic>1283G<\/jats:styled-content>\/T<\/jats:styled-content> (A<\/jats:styled-content>rg428L<\/jats:styled-content>eu) and KEL<\/jats:italic><\/jats:styled-content>*<\/jats:italic>1216C<\/jats:styled-content>\/T<\/jats:styled-content> (A<\/jats:styled-content>rg406S<\/jats:styled-content>top) in Exon 11. Red blood cells (RBCs<\/jats:styled-content>) from P<\/jats:styled-content>roband 1 and her sister were not agglutinated by plasma from P<\/jats:styled-content>roband 2; however, RBCs<\/jats:styled-content> from P<\/jats:styled-content>roband 2 were agglutinated by plasma from P<\/jats:styled-content>roband 1. P<\/jats:styled-content>robands 3, 4, 5, and 6 had the KEL*<\/jats:italic>1391C>T<\/jats:styled-content> change associated with the previously reported KETI<\/jats:styled-content>\u2013 phenotype. P<\/jats:styled-content>roband 5 was also homozygous for KEL<\/jats:italic><\/jats:styled-content>*905T<\/jats:styled-content>>C<\/jats:styled-content> encoding the K<\/jats:styled-content>11\u2013K<\/jats:styled-content>17+ phenotype. Hemagglutination studies revealed an association between KUCI<\/jats:styled-content>, KANT<\/jats:styled-content>, KETI<\/jats:styled-content>, and K<\/jats:styled-content>11. Protein modeling indicated that whereas A<\/jats:styled-content>la424 and A<\/jats:styled-content>rg428 are clustered, V<\/jats:styled-content>al302 and T<\/jats:styled-content>hr464 are not.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>A<\/jats:styled-content>la424 in the K<\/jats:styled-content>ell glycoprotein is associated with the high\u2010prevalence K<\/jats:styled-content>ell antigen, KUCI<\/jats:styled-content> (ISBT<\/jats:styled-content> 006032), which is detected by the antibody of P<\/jats:styled-content>roband 1. A<\/jats:styled-content>rg428 is associated with the high\u2010prevalence K<\/jats:styled-content>ell antigen, KANT<\/jats:styled-content> (ISBT<\/jats:styled-content> 006033). The association between KUCI<\/jats:styled-content>, KANT<\/jats:styled-content>, KETI<\/jats:styled-content>, and K<\/jats:styled-content>11 and the results of protein modeling are discussed.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12200","type":"journal-article","created":{"date-parts":[[2013,4,8]],"date-time":"2013-04-08T09:14:20Z","timestamp":1365412460000},"page":"2872-2881","source":"Crossref","is-referenced-by-count":8,"title":["Molecular basis of two novel and related high\u2010prevalence antigens in the K<\/scp>ell blood group system, KUCI<\/scp> and KANT<\/scp>, and their serologic and spatial association with K<\/scp>11 and KETI<\/scp>"],"prefix":"10.1111","volume":"53","author":[{"given":"Randall W.","family":"Velliquette","sequence":"first","affiliation":[{"name":"Laboratory of Immunohematology and Genomics New York Blood Center Long Island City New York"},{"name":"Laboratory of Immunochemistry New York Blood Center New York New York"},{"name":"Laboratory of Molecular 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