{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,23]],"date-time":"2026-04-23T23:39:26Z","timestamp":1776987566771,"version":"3.51.4"},"reference-count":6,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,6,30]],"date-time":"2013-06-30T00:00:00Z","timestamp":1372550400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,3]]},"abstract":"Case Report<\/jats:title>A 50\u2010year\u2010old male with T<\/jats:styled-content>\u2010cell lymphoma presented for autologous peripheral blood stem cell transplantation. After granulocyte\u2013colony\u2010stimulating factor (G\u2010CSF<\/jats:styled-content>) mobilization, his peripheral blood CD<\/jats:styled-content>34+ cell count was 166\u2009\u00d7\u2009106<\/jats:sup>\/L on the day before collection, which predicted a high yield of CD<\/jats:styled-content>34+ cells in the apheresis product. The first two collections had yields much lower than expected, triggering an investigation and changes to the apheresis collection methods since mobilization appeared adequate from the peripheral CD<\/jats:styled-content>34+ values.<\/jats:p><\/jats:sec>Results<\/jats:title>Changes to the apheresis collection variables and instrumentation did not improve the yields in the next three collections. The laboratory investigation demonstrated that there was an interfering substance in the patient's plasma that was causing falsely high peripheral blood CD<\/jats:styled-content>34+ cell values and that the low CD<\/jats:styled-content>34+ cell yields in the collections were consistent with the actual peripheral blood CD<\/jats:styled-content>34+ cell value. Based on this finding and after discussion with the clinical service, the patient then received plerixafor to increase the number of circulating CD<\/jats:styled-content>34+ cells before Collections 6 and 7. The patient went on to achieve the target CD<\/jats:styled-content>34+ cell dose and subsequently underwent a successful autologous transplant with full hematopoietic engraftment.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>This case demonstrates the importance of timely and critical review of laboratory results in the context of the specific patient. This case exemplifies how diligent review of laboratory results and open communication among the various teams can positively affect patient outcomes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12324","type":"journal-article","created":{"date-parts":[[2013,7,1]],"date-time":"2013-07-01T04:06:53Z","timestamp":1372651613000},"page":"541-544","source":"Crossref","is-referenced-by-count":3,"title":["Discordant CD<\/scp>34+ cell results in peripheral blood and hematopoietic progenitor cell\u2013apheresis product: implications for clinical decisions and impact on patient treatment"],"prefix":"10.1111","volume":"54","author":[{"given":"Courtney J.","family":"Liwski","sequence":"first","affiliation":[{"name":"Division of Transfusion Medicine, Department of Laboratory Medicine & Pathology Mayo Clinic Rochester Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas J.","family":"Padley","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Laboratory Medicine & Pathology Mayo Clinic Rochester Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Gustafson","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Laboratory Medicine & Pathology Mayo Clinic Rochester Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey L.","family":"Winters","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Laboratory Medicine & Pathology Mayo Clinic Rochester Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dennis A.","family":"Gastineau","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Laboratory Medicine & Pathology Mayo Clinic Rochester Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eapen K.","family":"Jacob","sequence":"additional","affiliation":[{"name":"Division of Transfusion Medicine, Department of Laboratory Medicine & Pathology Mayo Clinic Rochester Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,6,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2010.236"},{"key":"e_1_2_7_3_1","first-page":"148","article-title":"The negative effect of high peripheral white blood cell count on CD34+ cell recovery","volume":"17","author":"Burgstaler EA","year":"2002","journal-title":"J Clin Apher"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.20280"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1996.5.213"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jca.20011"},{"key":"e_1_2_7_7_1","first-page":"179","article-title":"A false expression of CD8 antigens on CD4+ T cells in a routine flow cytometry analysis","volume":"44","author":"Bukowska\u2010Strakova K","year":"2006","journal-title":"Folia Histochem Cytobiol"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.12324","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.12324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T14:35:14Z","timestamp":1696170914000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.12324"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,6,30]]},"references-count":6,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1111\/trf.12324"],"URL":"https:\/\/doi.org\/10.1111\/trf.12324","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,6,30]]}}}