{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:17:42Z","timestamp":1784247462167,"version":"3.55.0"},"reference-count":18,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,7,28]],"date-time":"2013-07-28T00:00:00Z","timestamp":1374969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Babesia<\/jats:italic><\/jats:styled-content> represents one of the major infectious threats to the blood supply since clinically silent infections in humans are common and these can be life\u2010threatening in certain recipients. It is important to understand the effect of blood storage conditions on the viability of <jats:styled-content style=\"fixed-case\"><jats:italic>Babesia<\/jats:italic><\/jats:styled-content> as this will impact the occurrence and severity of transfusion\u2010transmitted babesiosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Babesia divergens<\/jats:italic><\/jats:styled-content> was introduced into blood bags containing leukoreduced red blood cells (<jats:styled-content style=\"fixed-case\">RBCs<\/jats:styled-content>) and stored at 4\u00b0C for 0 to 31 days. Samples were withdrawn for assessment of the presence, morphology, and viability of parasites. Blood smears were made immediately on removal from blood bags at different time intervals and evaluated by blood film microscopy. <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s withdrawn from the bags were also cultured for 8 days using conditions optimal for parasite reproduction and growth to allow assessment of parasite viability.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>After 24 hours of storage at 4\u00b0C, there was a substantial reduction of parasitemia in the blood bags, which was maintained throughout storage. This decrease was accompanied by a change in morphology of parasites, with the number of altered parasites increasing through the period of storage. However, viability was maintained through 31 days of cold storage with a lag in achieving exponential growth seen in the parasites subjected to longer periods of refrigeration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Refrigeration of <jats:styled-content style=\"fixed-case\"><jats:italic>B.\u2009divergens<\/jats:italic><\/jats:styled-content> leads to an alteration of parasite morphology and a decrease in parasite numbers. However, there are sufficient parasites that are robust enough to survive 31 days of storage at 4\u00b0C and yield high end\u2010point parasitemia.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12357","type":"journal-article","created":{"date-parts":[[2013,7,29]],"date-time":"2013-07-29T04:46:30Z","timestamp":1375073190000},"page":"585-591","source":"Crossref","is-referenced-by-count":30,"title":["<scp><i>Babesia<\/i><\/scp>: impact of cold storage on the survival and the viability of parasites in blood bags"],"prefix":"10.1111","volume":"54","author":[{"given":"Jeny R.","family":"Cursino\u2010Santos","sequence":"first","affiliation":[{"name":"Department of Blood\u2010Borne Parasites, Lindsley Kimball Research Institute New York Blood Center  New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy","family":"Alhassan","sequence":"additional","affiliation":[{"name":"Department of Blood\u2010Borne Parasites, Lindsley Kimball Research Institute New York Blood Center  New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manpreet","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Blood\u2010Borne Parasites, Lindsley Kimball Research Institute New York Blood Center  New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheryl A.","family":"Lobo","sequence":"additional","affiliation":[{"name":"Department of Blood\u2010Borne Parasites, Lindsley Kimball Research Institute New York Blood Center  New York New York"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2006.00740.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.01.029"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-124-7-199604010-00004"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2006.03.008"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03467.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-155-8-201110180-00362"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02317.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12125"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02380.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-155-8-201110180-00363"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00022-10"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02872.x"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00934377"},{"key":"e_1_2_7_15_1","first-page":"505","article-title":"Babesiosis surveillance\u201418 States, 2011","volume":"61","author":"Centers for Disease Control and Prevention (CDC)","year":"2012","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03746.x"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2174\/187152611794407746"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2307\/3283978"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1991.31491213305.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.12357","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.12357","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T14:34:46Z","timestamp":1696170886000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.12357"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,28]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2014,3]]}},"alternative-id":["10.1111\/trf.12357"],"URL":"https:\/\/doi.org\/10.1111\/trf.12357","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,28]]}}}