{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T15:52:45Z","timestamp":1777650765099,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,9,4]],"date-time":"2013-09-04T00:00:00Z","timestamp":1378252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,4]]},"abstract":"Background<\/jats:title>Cultured red blood cells (cRBC<\/jats:styled-content>s) from cord blood (CB<\/jats:styled-content>) have been proposed as transfusion products. Whether buffy coats discarded from blood donations (adult blood [AB<\/jats:styled-content>]) may be used to generate cRBC<\/jats:styled-content>s for transfusion has not been investigated.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>Erythroid progenitor cell content and numbers and blood group antigen profiles of erythroblasts (ERYs<\/jats:styled-content>) and cRBC<\/jats:styled-content>s generated in human erythroid massive amplification (HEMA<\/jats:styled-content>) culture by CB<\/jats:styled-content> (n\u2009=\u20097) and AB<\/jats:styled-content> (n\u2009=\u200933, three females, three males, one AB<\/jats:styled-content> with rare blood antigens cryopreserved using CB<\/jats:styled-content> protocols) were compared.<\/jats:p><\/jats:sec>Results<\/jats:title>Variability was observed both in progenitor cell content (twofold) and number of ERYs<\/jats:styled-content> generated (1\u2009log) by CB<\/jats:styled-content> and AB<\/jats:styled-content> in HEMA<\/jats:styled-content>. The average progenitor cell contents of the subset of AB<\/jats:styled-content> and CB<\/jats:styled-content> analyzed were similar. AB<\/jats:styled-content> generated numbers of ERY<\/jats:styled-content>s three times lower (p\u2009<\u20090.01) than CB<\/jats:styled-content> in HEMA<\/jats:styled-content> containing fetal bovine serum but similar to CB<\/jats:styled-content> in HEMA<\/jats:styled-content> containing human proteins. Female AB<\/jats:styled-content> contained two times fewer (p\u2009<\u20090.05) erythroid progenitor cells but generated numbers of ERY<\/jats:styled-content>s similar to those generated by male AB<\/jats:styled-content>. Cryopreserved AB<\/jats:styled-content> with a rare blood group phenotype and shipped to another laboratory generated great numbers of ERY<\/jats:styled-content>s, 90% of which matured into cRBC<\/jats:styled-content>s. Blood group antigen expression was consistent with the donor genotype for ERY<\/jats:styled-content>s generated both by CB<\/jats:styled-content> and AB<\/jats:styled-content> but concordant with that of native RBC<\/jats:styled-content>s only for cells derived from AB<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Buffy coats from regular donors, including a donor with rare phenotypes stored under conditions established for CB<\/jats:styled-content>, are not inferior to CB<\/jats:styled-content> for the generation of cRBC<\/jats:styled-content>s.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12391","type":"journal-article","created":{"date-parts":[[2013,9,5]],"date-time":"2013-09-05T03:04:10Z","timestamp":1378350250000},"page":"1059-1070","source":"Crossref","is-referenced-by-count":11,"title":["Mononuclear cells from a rare blood donor, after freezing under good manufacturing practice conditions, generate red blood cells that recapitulate the rare blood phenotype"],"prefix":"10.1111","volume":"54","author":[{"given":"Francesca","family":"Masiello","sequence":"first","affiliation":[{"name":"Hematology\/Oncology and Molecular Medicine Istituto Superiore di Sanita' Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Valentina","family":"Tirelli","sequence":"additional","affiliation":[{"name":"Hematology\/Oncology and Molecular Medicine Istituto Superiore di Sanita' Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Massimo","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Cell Biology and Neuroscience Istituto Superiore di Sanita' Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emile","family":"van den Akker","sequence":"additional","affiliation":[{"name":"Sanquin Blood Supply Amsterdam the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Girelli","family":"Gabriella","sequence":"additional","affiliation":[{"name":"Centro Trasfusionale Universita' La Sapienza Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Marconi","sequence":"additional","affiliation":[{"name":"Centro Trasfusionale e di Immunoematologia, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Antonietta","family":"Villa","sequence":"additional","affiliation":[{"name":"Centro Trasfusionale e di Immunoematologia, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paolo","family":"Rebulla","sequence":"additional","affiliation":[{"name":"Centro Trasfusionale e di Immunoematologia, Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghazala","family":"Hashmi","sequence":"additional","affiliation":[{"name":"MihLabs Warren New Jersey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolyn","family":"Whitsett","sequence":"additional","affiliation":[{"name":"Kings County Hospital and Downstate Medical Center Brooklyn New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna Rita","family":"Migliaccio","sequence":"additional","affiliation":[{"name":"Hematology\/Oncology and Molecular Medicine Istituto Superiore di Sanita' Rome Italy"},{"name":"Tisch Cancer Institute Mount Sinai School of Medicine New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,9,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00439-009-0738-2"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e3283316bed"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-07-077370"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.03024.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12146"},{"key":"e_1_2_8_7_1","first-page":"2","article-title":"Rare gems: null phenotypes and blood groups","volume":"8","author":"Flegel WA","year":"2010","journal-title":"Blood Transfus"},{"key":"e_1_2_8_8_1","unstructured":"Food and Drug Administration.Alphabetical list of licensed establishments including product approval dates information updated through July 31 2013. 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