{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T23:15:36Z","timestamp":1784157336248,"version":"3.55.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T00:00:00Z","timestamp":1392595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Autologous stem cell transplants in patients with hemoglobinopathies are limited. Previous reports used granulocyte\u2013colony\u2010stimulating factor (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>) for mobilization of stem cells; there are no reported cases undergoing plerixafor mobilization. We report such a patient, providing guidance for peripheral blood stem cells collection when aberrant red blood cells (<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s) disrupt normal separation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>A patient with \u03b2\u2010thalassemia intermedia and hereditary persistence of fetal hemoglobin presented for peripheral blood stem cell collection for autologous transplant for myeloma. He underwent splenectomy for anemia secondary to hemoglobinopathy and chemotherapy, ceasing<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>transfusions. The patient was mobilized using plerixafor after collection with<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>failed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Collections were performed using an apheresis system, processing 24\u2009<jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>daily. Peripheral blood and apheresis product<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 determinations were performed daily. On<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content>ay\u20091, the product yield was 0.04\u2009\u00d7\u200910<jats:sup>6<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 cells\/kg, less than expected based on white blood cell count and<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34\u2010positive cells. Peripheral blood smear showed nucleated<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>s and<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>morphologic abnormalities. Changes in instrument variables were made after consultation with<jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>erumo<jats:styled-content style=\"fixed-case\">BCT<\/jats:styled-content>to adjust for variable distribution of mononuclear and stem cells during centrifugation. Collecting stem cells at a deeper location and centrifuging faster improved collection, and a cumulative total of 4.40\u2009\u00d7\u200910<jats:sup>6<\/jats:sup><jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 cells\/kg was achieved after four collections. The patient underwent tandem autologous transplantation and engrafted within 12 to 13 days of each transplant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Adjustments in apheresis variables allowed successful collection of peripheral blood stem cells from a patient with<jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>anomalies of \u03b2\u2010thalassemia that interfered with standard stem cell harvesting.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12579","type":"journal-article","created":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T12:16:46Z","timestamp":1392639406000},"page":"1881-1886","source":"Crossref","is-referenced-by-count":10,"title":["Harvesting autologous stem cells from a patient with red blood cell abnormalities of \u03b2\u2010thalassemia intermedia"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7725-6854","authenticated-orcid":false,"given":"Kimberly","family":"Sanford","sequence":"first","affiliation":[{"name":"Department of Pathology Virginia Commonwealth University Richmond Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan D.","family":"Roseff","sequence":"additional","affiliation":[{"name":"Department of Pathology Virginia Commonwealth University Richmond Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Anderson","sequence":"additional","affiliation":[{"name":"Department of Pathology Virginia Commonwealth University Richmond Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harold M.","family":"Chung","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Virginia Commonwealth University Richmond Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard A.","family":"McPherson","sequence":"additional","affiliation":[{"name":"Department of Pathology Virginia Commonwealth University Richmond Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.1.1996.5.213"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(82)90319-1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199002153220701"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199309163291204"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.045"},{"key":"e_1_2_8_7_1","first-page":"1187","article-title":"Survival and complications in patients with thalassemia major treated with transfusion and 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