{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T12:12:50Z","timestamp":1769688770718,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T00:00:00Z","timestamp":1395705600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Macopharma"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>abesia<\/jats:italic> spp. is an intraerythrocytic parasite that causes human babesiosis and its transmission by transfusion has been extensively demonstrated. The aim of this study was to ascertain the efficacy of an ultraviolet\u2009<jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">UVC<\/jats:styled-content>)\u2010based pathogen inactivation system in the reduction of <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>abesia\u2009divergens<\/jats:italic>\u2013infected platelet (<jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>) concentrates and to determine the parasite's ability to survive in <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content> concentrates stored under blood bank conditions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>This study was conducted using in vitro cultures of <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009divergens<\/jats:italic>. The detection limit of the culture assay was established and, subsequently, 15 buffy coat\u2013derived <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content> concentrates (<jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s) were inoculated with 10<jats:sup>7<\/jats:sup> <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009divergens<\/jats:italic>\u2013infected red blood cells. Infected <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s were irradiated with 0.2\u2009<jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>\/cm<jats:sup>2<\/jats:sup> <jats:styled-content style=\"fixed-case\">UVC<\/jats:styled-content> light using the <jats:styled-content style=\"fixed-case\">THERAFLEX UV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>latelets method (<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>acopharma). Viability and parasite growth were evaluated before and after inactivation. Culture growth kinetics were monitored by <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> incorporation of [<jats:sup>3<\/jats:sup><jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>]thymidine. The ability of <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009divergens<\/jats:italic> to survive in <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content> concentrates was also analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The limit of detection in cultures was established at 0.1\u2009\u00d7\u200910<jats:sup>\u22126<\/jats:sup>% parasites. The <jats:styled-content style=\"fixed-case\">THERAFLEX UV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">P<\/jats:styled-content>latelets system inactivated <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009divergens<\/jats:italic> to below the limit of detection in 12 of 15 <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s (log reduction, &gt;6.0) and to the limit of detection (log reduction, 5.0) in three of 15. It was also demonstrated that <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009divergens<\/jats:italic> remains viable in <jats:styled-content style=\"fixed-case\">BC<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>s stored up to 7 days.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Since <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009divergens<\/jats:italic> can survive in <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content> concentrates and given the performance of <jats:styled-content style=\"fixed-case\">UVC<\/jats:styled-content>, this system could be considered as an alternative to prevent <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>.\u2009divergens<\/jats:italic> and other <jats:italic><jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>abesia<\/jats:italic> species from being transmitted through <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content> transfusions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12598","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T09:36:06Z","timestamp":1395740166000},"page":"2207-2216","source":"Crossref","is-referenced-by-count":26,"title":["The efficacy of the ultraviolet\u2009<scp>C<\/scp> pathogen inactivation system in the reduction of <i><scp>B<\/scp>abesia divergens<\/i> in pooled buffy coat platelets"],"prefix":"10.1111","volume":"54","author":[{"given":"Emma","family":"Castro","sequence":"first","affiliation":[{"name":"Centro de Transfusi\u00f3n de Cruz Roja Espa\u00f1ola en Madrid  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis Miguel","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Servicio de Parasitolog\u00eda Centro Nacional de Microbiolog\u00eda Instituto de Salud Carlos III  Madrid 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