{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T19:36:07Z","timestamp":1782934567217,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T00:00:00Z","timestamp":1397433600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Antibiotic prophylaxis treatment at delivery is highly recommended for reducing the risk of infection for mothers positive for group <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> streptococcus. It is therefore expected that some cord blood (<jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content>) products will contain residual antibiotics. This study aimed to determine the incidence and level of \u03b2\u2010lactam antibiotics in <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> products.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>The antimicrobial activity of 60 <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> plasma by\u2010products was evaluated using disk diffusion assays on 10 bacteria species. Plasma samples showing antimicrobial activity were either treated with \u03b2\u2010lactamase enzyme to inhibit \u03b2\u2010lactam antibiotics or heated to 56\u00b0C for 30 minutes to inhibit complement proteins. \u03b2\u2010Lactam antibiotic concentrations were determined by comparison with a standard curve obtained with known concentrations of antibiotics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Antimicrobial activity against mostly Gram\u2010positive microorganisms was observed in 33% of <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> units. The \u03b2\u2010lactamase enzyme abolished the antimicrobial activity in the majority of these <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> products. Up to 5\u2009\u03bcg\/mL penicillin and 14\u2009\u03bcg\/mL ampicillin were measured in these products.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Approximately one\u2010third of <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> products contain significant amounts of plasma with residual antibiotics, which can affect the survival and growth of bacterial contaminants when performing the sterility test and potentially lead to false\u2010negative results. Additional work is required to better understand whether residual antibiotics in <jats:styled-content style=\"fixed-case\">CB<\/jats:styled-content> affect penicillin\u2010allergic patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12655","type":"journal-article","created":{"date-parts":[[2014,4,14]],"date-time":"2014-04-14T07:36:43Z","timestamp":1397461003000},"page":"2505-2513","source":"Crossref","is-referenced-by-count":7,"title":["Antimicrobial activity in cord blood units: occurrence and levels of antibiotics"],"prefix":"10.1111","volume":"54","author":[{"given":"Marie\u2010Pierre","family":"Cayer","sequence":"first","affiliation":[{"name":"Research and Development H\u00e9ma\u2010Qu\u00e9bec  Quebec Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lissa","family":"Girard","sequence":"additional","affiliation":[{"name":"Research and Development H\u00e9ma\u2010Qu\u00e9bec  Quebec Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane","family":"Fournier","sequence":"additional","affiliation":[{"name":"Public Cord Blood Bank H\u00e9ma\u2010Qu\u00e9bec  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Delage","sequence":"additional","affiliation":[{"name":"Medical Affairs H\u00e9ma\u2010Qu\u00e9bec  Montreal Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Thibault","sequence":"additional","affiliation":[{"name":"Research and Development H\u00e9ma\u2010Qu\u00e9bec  Quebec Quebec Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,4,14]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Food and Drug Administration.Minimally manipulated unrelated allogeneic placental\/umbilical cord blood intended for hematopoietic reconstitution for specified indications. 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