{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:20:20Z","timestamp":1773012020958,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T00:00:00Z","timestamp":1399507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Background<\/jats:title>Nucleic acid test (NAT<\/jats:styled-content>) hepatitis\u2009B virus (HBV<\/jats:styled-content>) screening for all blood donations with a sensitivity limit of 25\u2009IU<\/jats:styled-content>\/mL<\/jats:styled-content> in the individual donation is mandatory in S<\/jats:styled-content>witzerland since 2009. The aims of the two studies were to define the percentage of antibody to hepatitis\u2009B<\/jats:styled-content> core antigen (anti\u2010HB<\/jats:styled-content>c) or anti\u2010HB<\/jats:styled-content>c and antibody to hepatitis\u2009B<\/jats:styled-content> surface antigen (anti\u2010HB<\/jats:styled-content>s)\u2010positive donors bearing HBV DNA<\/jats:styled-content> and to gather HBV<\/jats:styled-content> viral load data on HBV NAT<\/jats:styled-content> yields during the routine screening since the introduction of the HBV NAT<\/jats:styled-content>.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>Archive samples from anti\u2010HB<\/jats:styled-content>c\u2013positive donors (G<\/jats:styled-content>roup I<\/jats:styled-content>) were analyzed with a quantitative HBV DNA<\/jats:styled-content> test and further with anti\u2010HB<\/jats:styled-content>c and anti\u2010HB<\/jats:styled-content>s assays. In addition, all the HBV NAT<\/jats:styled-content>\u2010only\u2013yield samples (G<\/jats:styled-content>roup II<\/jats:styled-content>) from the routine donor screening performed between J<\/jats:styled-content>uly 2007 and M<\/jats:styled-content>ay 2013 were included in the study.<\/jats:p><\/jats:sec>Results<\/jats:title>From the 667 samples investigated (131 donors), three donors (2.3%) had donated eight samples (1.2%) with detectable HBV DNA<\/jats:styled-content>; however, all had very low viral loads (\u226410\u2009IU<\/jats:styled-content>\/mL<\/jats:styled-content>). From the 1,160,426 donations screened with the routine HBV NAT<\/jats:styled-content> assay, 16 HBV NAT<\/jats:styled-content> yields were detected: two window period (WP<\/jats:styled-content>) and 14 occult hepatitis B<\/jats:styled-content> infection (OBI<\/jats:styled-content>) cases. In eight of these positive donations (two WP<\/jats:styled-content> and six OBI<\/jats:styled-content>), the HBV<\/jats:styled-content> viral loads were not more than 10\u2009IU<\/jats:styled-content>\/mL<\/jats:styled-content>, in three cases between 10 and 25\u2009IU<\/jats:styled-content>\/mL<\/jats:styled-content>, and in the remaining five donations between 37\u2009and 166\u2009IU<\/jats:styled-content>\/mL<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>The highly sensitive HBV NAT<\/jats:styled-content> assay with a threshold significantly below 10\u2009IU<\/jats:styled-content>\/mL<\/jats:styled-content> is a valuable alternative to anti\u2010HB<\/jats:styled-content>c and a less sensitive HBV NAT<\/jats:styled-content> screening in blood donor screening.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12694","type":"journal-article","created":{"date-parts":[[2014,5,8]],"date-time":"2014-05-08T08:27:40Z","timestamp":1399537660000},"page":"2961-2967","source":"Crossref","is-referenced-by-count":13,"title":["H<\/scp>epatitis B<\/scp> virus DNA<\/scp> viral load determination in hepatitis\u2009B<\/scp> surface antigen\u2013negative S<\/scp>wiss blood donors"],"prefix":"10.1111","volume":"54","author":[{"given":"Martin","family":"Stolz","sequence":"first","affiliation":[{"name":"Blood Transfusion Service SRC Berne Berne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Tinguely","sequence":"additional","affiliation":[{"name":"Blood Transfusion Service SRC Berne Berne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Fontana","sequence":"additional","affiliation":[{"name":"Blood Transfusion Service SRC Berne Berne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Niederhauser","sequence":"additional","affiliation":[{"name":"Blood Transfusion Service SRC Berne Berne Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,8]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"SteblerC InfantiL EicheB et\u2009al.Prevalence of anti\u2010HBc antibodies in Swiss blood donors a 4 month survey at the Blood Transfusion Centre Basel. 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