{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T07:01:57Z","timestamp":1790146917567,"version":"4.0.1"},"reference-count":59,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,6,4]],"date-time":"2014-06-04T00:00:00Z","timestamp":1401840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Background<\/jats:title>Red blood cells (RBCs<\/jats:styled-content>) with D<\/jats:styled-content> antigen levels only detected by anti\u2010D<\/jats:styled-content> adsorption\u2010elution and an antiglobulin test express a DEL<\/jats:styled-content> phenotype. For two DEL<\/jats:styled-content> types, including RHD<\/jats:styled-content>(1227G<\/jats:styled-content><\/jats:italic>>A<\/jats:styled-content>)<\/jats:italic>, immunization of D<\/jats:styled-content>\u2013 recipients has been reported. This study's aim was to measure the prevalence of DEL<\/jats:styled-content>\u2010associated RHD<\/jats:styled-content><\/jats:italic> alleles in a cohort of A<\/jats:styled-content>ustralian D<\/jats:styled-content>\u2013 donors to develop a model to estimate alloimmunization risk.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>D<\/jats:styled-content>\u2013, C<\/jats:styled-content>+ and\/or E<\/jats:styled-content>+ blood donors were screened for RHD<\/jats:styled-content><\/jats:italic> exons using quantitative polymerase chain reaction. Donors with RHD<\/jats:styled-content><\/jats:italic> signals were DEL<\/jats:styled-content> phenotyped with MCAD<\/jats:styled-content>6 anti\u2010D<\/jats:styled-content>. RHD<\/jats:styled-content><\/jats:italic> alleles were characterized via single\u2010nucleotide polymorphism array or sequencing. Extended DEL<\/jats:styled-content> phenotyping was performed with an anti\u2010D<\/jats:styled-content> panel.<\/jats:p><\/jats:sec>Results<\/jats:title>Among 2027 donors, 39 carried RHD<\/jats:styled-content><\/jats:italic> alleles that have been previously reported to associate with either the DEL<\/jats:styled-content> or the weak D<\/jats:styled-content> phenotype. An additional five donors carried previously unreported RHD<\/jats:styled-content><\/jats:italic> alleles and exhibited the DEL<\/jats:styled-content> phenotype: RHD<\/jats:styled-content>(IVS<\/jats:styled-content>2\u20102delA<\/jats:styled-content>)<\/jats:italic>, RHD<\/jats:styled-content>(IVS<\/jats:styled-content>1<\/jats:italic>+5G<\/jats:styled-content><\/jats:italic>>C<\/jats:styled-content>)<\/jats:italic>, RHD<\/jats:styled-content>(ex9:del\/CE<\/jats:styled-content>)<\/jats:italic>, and RHD<\/jats:styled-content>(ex8:del\/CE<\/jats:styled-content>)<\/jats:italic> represented twice. In total, DEL<\/jats:styled-content>\/weak D<\/jats:styled-content>\u2013associated RHD<\/jats:italic> alleles were detected in 44 of 2027 donors or 2.17% (95% confidence interval, 1.54%\u20102.81%). The RHD<\/jats:styled-content>(1227G<\/jats:styled-content><\/jats:italic>>A<\/jats:styled-content>)<\/jats:italic>\u2005DEL<\/jats:styled-content> allele was the most frequent (n\u2009=\u200916). The risk of transfusing D<\/jats:styled-content>\u2013 females not more than 40 years of age with an RHD<\/jats:styled-content>(1227G<\/jats:styled-content><\/jats:italic>>A<\/jats:styled-content>)<\/jats:italic>\u2005DEL RBC<\/jats:styled-content> unit (when managed as D<\/jats:styled-content>\u2013) is estimated to be one in 149,109 transfusions (range, 100,680\u2010294,490).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>DEL<\/jats:styled-content>\/weak D<\/jats:styled-content>\u2013associated RHD<\/jats:styled-content><\/jats:italic> alleles were found in 2.17% of A<\/jats:styled-content>ustralian D<\/jats:styled-content>\u2013, C<\/jats:styled-content>+ and\/or E<\/jats:styled-content>+ blood donors. This differs from previous E<\/jats:styled-content>uropean reports in that the clinically significant RHD<\/jats:styled-content>(1227G<\/jats:styled-content><\/jats:italic>>A<\/jats:styled-content>)<\/jats:italic>\u2005DEL<\/jats:styled-content> allele is the most prevalent.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12701","type":"journal-article","created":{"date-parts":[[2014,6,4]],"date-time":"2014-06-04T11:16:27Z","timestamp":1401880587000},"page":"2931-2940","source":"Crossref","is-referenced-by-count":28,"title":["The RHD<\/scp>(1227G<\/scp><\/i>>A<\/scp>)<\/i>\u2005DEL<\/scp>\u2010associated allele is the most prevalent DEL<\/scp> allele in A<\/scp>ustralian D<\/scp>\u2013 blood donors with C<\/scp>+ and\/or E<\/scp>+ phenotypes"],"prefix":"10.1111","volume":"54","author":[{"given":"Stacy A.","family":"Scott","sequence":"first","affiliation":[{"name":"Research and Development Australian Red Cross Blood Service Brisbane 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Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Flower","sequence":"additional","affiliation":[{"name":"Research and Development Australian Red Cross Blood Service Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Hyland","sequence":"additional","affiliation":[{"name":"Research and Development Australian Red Cross Blood Service Brisbane Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,6,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V95.2.375"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-217"},{"key":"e_1_2_8_4_1","volume-title":"Chapter 5: Rh blood group system","author":"Daniels 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