{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T02:13:37Z","timestamp":1779243217295,"version":"3.51.4"},"reference-count":49,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,5,19]],"date-time":"2014-05-19T00:00:00Z","timestamp":1400457600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["K08-HL103756"],"award-info":[{"award-number":["K08-HL103756"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U01-HD064827"],"award-info":[{"award-number":["U01-HD064827"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Louis V. Gerstner Scholars Award"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2014,11]]},"abstract":"Background<\/jats:title>Although human red blood cell (RBC<\/jats:styled-content>) units may be refrigerator stored for up to 42 days, transfusion of older RBC<\/jats:styled-content>s acutely delivers a large bolus of iron to mononuclear phagocytes. Similarly, iron dextran circulates in plasma for hours to days and is progressively cleared by mononuclear phagocytes, which return iron to plasma. Finally, malaria infection continuously delivers iron to macrophages by intra\u2010 and extravascular hemolysis. Studies suggest that iron administration increases infectious risk.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>To assess the effects of increased iron availability on susceptibility to infection, we infected mice with model G<\/jats:styled-content>ram\u2010negative intracellular or extracellular pathogens (S<\/jats:styled-content>almonella typhimurium<\/jats:italic> or E<\/jats:styled-content>scherichia coli<\/jats:italic>, respectively), accompanied by RBC<\/jats:styled-content> transfusion, iron dextran administration, or malarial coinfection.<\/jats:p><\/jats:sec>Results<\/jats:title>In our mouse models, transfusion of older RBC<\/jats:styled-content>s exacerbates infection with both G<\/jats:styled-content>ram\u2010negative pathogens. Although iron dextran exacerbates E<\/jats:styled-content>.\u2009coli<\/jats:italic> infection to a similar extent as transfusion of corresponding amounts of iron, higher iron doses are required to produce comparable effects with S<\/jats:styled-content>.\u2009typhimurium<\/jats:italic>. Coinfection of mice with P<\/jats:styled-content>lasmodium yoelii<\/jats:italic> and S<\/jats:styled-content>.\u2009typhimurium<\/jats:italic> produces overwhelming S<\/jats:styled-content>almonella<\/jats:italic> sepsis. Finally, treating mice with antibiotics abrogates the enhancing effect on E<\/jats:styled-content>.\u2009coli<\/jats:italic> infection of both older RBC<\/jats:styled-content> transfusion and iron dextran administration.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Transfusion of older RBC<\/jats:styled-content>s exacerbates G<\/jats:styled-content>ram\u2010negative infection to a similar extent as malaria coinfection or iron dextran administration. Appropriate antibiotic therapy abrogates the effect of older RBC<\/jats:styled-content> transfusions on infection with E<\/jats:styled-content>.\u2009coli.<\/jats:italic> Iron delivery to macrophages may be an underappreciated mechanism mediating, at least some, adverse effects of RBC<\/jats:styled-content> transfusions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12712","type":"journal-article","created":{"date-parts":[[2014,5,20]],"date-time":"2014-05-20T03:00:57Z","timestamp":1400554857000},"page":"2842-2851","source":"Crossref","is-referenced-by-count":47,"title":["Transfusion of stored blood impairs host defenses against G<\/scp>ram\u2010negative pathogens in mice"],"prefix":"10.1111","volume":"54","author":[{"given":"Kevin","family":"Prestia","sequence":"first","affiliation":[{"name":"Institute of Comparative Medicine Columbia University Medical Center\u2013New York Presbyterian Hospital New York New York"},{"name":"Department of Pathology and Cell Biology Columbia University 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