{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:48:55Z","timestamp":1788871735314,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T00:00:00Z","timestamp":1411430400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007055","name":"Third Military Medical University","doi-asserted-by":"crossref","award":["2010XLC23"],"award-info":[{"award-number":["2010XLC23"]}],"id":[{"id":"10.13039\/501100007055","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The induction of hematopoietic stem cells to produce mass numbers of platelets (\n                      <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>\n                      s) in vitro is an effective method to address a lack of\n                      <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>\n                      s and\n                      <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>\n                      transfusion resistance in the clinic. However, the design of a low\u2010cost and sustainable culture system is currently problematic.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>\n                      Here, the thrombopoietin, interleukin (\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      )\u20106, and\n                      <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\n                      \u201011 genes, three regulatory factors important for thrombopoiesis, were used to construct self\u2010splicing fusion genes linked by foot and mouth disease (\n                      <jats:styled-content style=\"fixed-case\">F2A<\/jats:styled-content>\n                      ) and Theiler's murine encephalitis (\n                      <jats:styled-content style=\"fixed-case\">T2A<\/jats:styled-content>\n                      ) viruses. Bone marrow stromal cells (\n                      <jats:styled-content style=\"fixed-case\">BMSCs<\/jats:styled-content>\n                      ) transduced with the fusion gene acted as nourishing cells and induced cord blood mononuclear cells (\n                      <jats:styled-content style=\"fixed-case\">MNC<\/jats:styled-content>\n                      s) to generate\n                      <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>\n                      s in vitro. We counted these cells; determined the percentage of cells expressing specific cell surface markers (\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      41); and measured their ability to aggregate via flow cytometry, immunohistochemical staining, and aggregation remote analyzer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      <jats:styled-content style=\"fixed-case\">BMSC<\/jats:styled-content>\n                      s transduced with the fusion gene successfully induced cord blood\n                      <jats:styled-content style=\"fixed-case\">MNCs<\/jats:styled-content>\n                      to generate\n                      <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>\n                      \u2010sized fragments in the absence of exogenous cytokines. The output was higher than that of the control groups, and the\n                      <jats:styled-content style=\"fixed-case\">PLT<\/jats:styled-content>\n                      \u2010sized fragments were similar to endogenous\n                      <jats:styled-content style=\"fixed-case\">PLTs<\/jats:styled-content>\n                      in terms of shape,\n                      <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                      41 expression, and aggregation function.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      These results suggest that our method could be used to develop a low\u2010cost sustainable cultivation system that generates\n                      <jats:styled-content style=\"fixed-case\">PLTs<\/jats:styled-content>\n                      in vitro by enhancing the autocrine production of related cytokines through the nourishment provided by cells transduced with a syncretic gene.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.12800","type":"journal-article","created":{"date-parts":[[2014,9,23]],"date-time":"2014-09-23T22:28:42Z","timestamp":1411511322000},"page":"176-186","source":"Crossref","is-referenced-by-count":6,"title":["Bone marrow stromal cells transduced with a thrombopoietin, interleukin\u20106, and interleukin\u201011 syncretic gene induce cord mononuclear cells to generate platelets in vitro"],"prefix":"10.1111","volume":"55","author":[{"given":"Hua","family":"Lu","sequence":"first","affiliation":[{"name":"Transfusion Department Southwest Hospital Third Military Medical University Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianlun","family":"Jiang","sequence":"additional","affiliation":[{"name":"Transfusion Department Southwest Hospital Third Military Medical 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