{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:23:36Z","timestamp":1791296616789,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,1]],"date-time":"2014-09-01T00:00:00Z","timestamp":1409529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,2]]},"abstract":"Background<\/jats:title>Leukofiltration of blood components is currently implemented worldwide as a precautionary measure against white blood cell\u2013associated adverse effects and the potential transmission of variant C<\/jats:styled-content>reutzfeldt\u2010J<\/jats:styled-content>akob disease (vCJD<\/jats:styled-content>). A newly developed bifunctional filter (S<\/jats:styled-content>epacell P<\/jats:styled-content>rima, A<\/jats:styled-content>sahi K<\/jats:styled-content>asei M<\/jats:styled-content>edical) was assessed for prion removal, leukoreduction (LR<\/jats:styled-content>), and whether the filter significantly affected red blood cells (RBC<\/jats:styled-content>s).<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>S<\/jats:styled-content>epacell P<\/jats:styled-content>rima's postfiltration effects on RBC<\/jats:styled-content>s, including hemolysis, complement activation, and RBC<\/jats:styled-content> chemistry, were compared with those of a conventional LR<\/jats:styled-content> filter (S<\/jats:styled-content>epacell P<\/jats:styled-content>ure RC<\/jats:styled-content>). Prion removal was measured by W<\/jats:styled-content>estern blot after spiking RBC<\/jats:styled-content>s with microsomal fractions derived from scrapie\u2010infected hamster brain homogenate. Serially diluted exogenous prion solutions (0.05\u2009mL<\/jats:styled-content>), with or without filtration, were injected intracerebrally into Golden S<\/jats:styled-content>yrian hamsters.<\/jats:p><\/jats:sec>Results<\/jats:title>LR<\/jats:styled-content> efficiency of 4.44 log with the S<\/jats:styled-content>epacell P<\/jats:styled-content>rima was comparable to 4.11 log with the conventional LR<\/jats:styled-content> filter. There were no significant differences between the two filters in hemoglobin loss, hemolysis, complement activation, and RBC<\/jats:styled-content> biomarkers. In vitro reduction of exogenously spiked prions by the filter exceeded 3 log. The titer, 6.63 (log ID<\/jats:styled-content>50<\/jats:sub>\/mL), of prefiltration infectivity of healthy hamsters was reduced to 2.52 (log ID<\/jats:styled-content>50<\/jats:sub>\/mL) after filtration. The reduction factor was calculated as 4.20 (log ID<\/jats:styled-content>50<\/jats:sub>).<\/jats:p><\/jats:sec>Conclusion<\/jats:title>With confirmed removal efficacy for exogenous prion protein, this new bifunctional prion and LR<\/jats:styled-content> filter should reduce the residual risk of vCJD<\/jats:styled-content> transmission through blood transfusion without adding complexity to component processing.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12839","type":"journal-article","created":{"date-parts":[[2014,9,2]],"date-time":"2014-09-02T04:10:23Z","timestamp":1409631023000},"page":"330-336","source":"Crossref","is-referenced-by-count":2,"title":["Development of a bifunctional filter for prion protein and leukoreduction of red blood cell components"],"prefix":"10.1111","volume":"55","author":[{"given":"Tomo","family":"Yokomizo","sequence":"first","affiliation":[{"name":"Sepacell Development Planning Department Asahi Kasei Medical Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takako","family":"Kai","sequence":"additional","affiliation":[{"name":"Sepacell Development Planning Department Asahi Kasei Medical Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Morikazu","family":"Miura","sequence":"additional","affiliation":[{"name":"Sepacell Development Planning Department Asahi Kasei Medical Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Ohto","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion and Transplantation Immunology School of Medicine Fukushima Medical University Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15486-X"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16811-6"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38998408999.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2009.02500.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042019"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16812-8"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.2009.00918.x"},{"key":"e_1_2_8_9_1","first-page":"791","article-title":"Effect of anti\u2010coagulants on prestorage leukocyte\u2010reduced red cell components: CPD vs ACD\u2010A solution","volume":"47","author":"Akino M","year":"2001","journal-title":"Jpn J Transfus Med"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(99)00135-4"},{"key":"e_1_2_8_11_1","first-page":"48","volume-title":"Diagnostic procedures for viral and rickettsial disease","author":"Lennette EH","year":"1964"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9975(68)90006-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2006.00765.x"},{"key":"e_1_2_8_14_1","first-page":"86A","article-title":"A combination filter for prion and leukocyte reduction, its robustness to blood product variation","volume":"48","author":"Yokomizo T","year":"2008","journal-title":"Transfusion"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)24035-9"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03199.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.f5675"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12369"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.12839","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.12839","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T20:53:38Z","timestamp":1695502418000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.12839"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,9]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2015,2]]}},"alternative-id":["10.1111\/trf.12839"],"URL":"https:\/\/doi.org\/10.1111\/trf.12839","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,9]]}}}