{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T13:58:54Z","timestamp":1782482334299,"version":"3.54.5"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T00:00:00Z","timestamp":1411084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Guangdong Provincial Science and Technology Foundation","award":["2010B031000024 and 2011B090400407"],"award-info":[{"award-number":["2010B031000024 and 2011B090400407"]}]},{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2010CB530204"],"award-info":[{"award-number":["2010CB530204"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["31100657 and 31372443"],"award-info":[{"award-number":["31100657 and 31372443"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,3]]},"abstract":"Background<\/jats:title>Brucellosis is a severe zoonotic disease that is increasingly prevalent in north C<\/jats:styled-content>hina. A study evaluating B<\/jats:styled-content>rucella<\/jats:italic> infection in blood donors was conducted at K<\/jats:styled-content>ashi central blood station, X<\/jats:styled-content>injiang, C<\/jats:styled-content>hina.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>Four serologic and two molecular methods of detection of B<\/jats:styled-content>rucella<\/jats:italic> infection were used in plasma samples from blood donations collected from K<\/jats:styled-content>ashi in northwest C<\/jats:styled-content>hina, considered a brucellosis\u2010endemic area. Blood donor samples collected in S<\/jats:styled-content>henzhen, southern C<\/jats:styled-content>hina, a brucellosis\u2010nonendemic area, were tested as a negative control group.<\/jats:p><\/jats:sec>Results<\/jats:title>In 3896 plasma samples collected from K<\/jats:styled-content>ashi central blood station, 135 (3.5%) plasma samples were reactive by the R<\/jats:styled-content>ose B<\/jats:styled-content>engal plate test (RBPT<\/jats:styled-content>), and 120 (3.1%) of the 135 RBPT<\/jats:styled-content>\u2010reactive sample were also reactive with the standard tube agglutination test (SAT<\/jats:styled-content>), respectively. All samples of the control group of 399 blood samples from S<\/jats:styled-content>henzhen blood center tested negative with RBPT<\/jats:styled-content> and SAT<\/jats:styled-content>. Of 135 seroreactive plasma samples, 39 (1.0%) reacted with B<\/jats:styled-content>.\u2009melitensis<\/jats:italic> membrane protein extracts by enzyme\u2010linked immunosorbent assay and 25 were reactive to either rBP<\/jats:styled-content>26 or rOMP<\/jats:styled-content>31 by W<\/jats:styled-content>estern blot. Thirteen plasma samples and two follow\u2010up blood samples were identified as carrying B<\/jats:styled-content>rucella<\/jats:italic>\u2005DNA<\/jats:styled-content> by quantitative polymerase chain reaction (PCR<\/jats:styled-content>) and nested PCR<\/jats:styled-content>. Overall 15 (1:300) K<\/jats:styled-content>ashi blood donations were found positive by nucleic acid testing, confirmed specific by DNA<\/jats:styled-content> sequencing.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The data indicate a probable high rate of B<\/jats:styled-content>rucella<\/jats:italic> bacteremia, suggesting a potential risk of transfusion\u2010transmitted brucellosis. Blood donation screening for B<\/jats:styled-content>rucella<\/jats:italic> infection may be considered in the high B<\/jats:styled-content>rucella<\/jats:italic>\u2010endemic areas of C<\/jats:styled-content>hina.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12853","type":"journal-article","created":{"date-parts":[[2014,9,19]],"date-time":"2014-09-19T10:30:26Z","timestamp":1411122626000},"page":"586-592","source":"Crossref","is-referenced-by-count":26,"title":["Potential risk of blood transfusion\u2010transmitted brucellosis in an endemic area of C<\/scp>hina"],"prefix":"10.1111","volume":"55","author":[{"given":"Wenjing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingyu","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Southern Medical University Guangzhou 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