{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T23:53:23Z","timestamp":1790466803612,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,11,2]],"date-time":"2014-11-02T00:00:00Z","timestamp":1414886400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Recently, <jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>apanese <jats:styled-content style=\"fixed-case\">R<\/jats:styled-content>ed <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>ross blood centers have changed the confirmatory test method from an indirect immunofluorescence (<jats:styled-content style=\"fixed-case\">IF<\/jats:styled-content>) technique to <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>estern blotting (<jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>) for antibodies against human <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell leukemia virus <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>ype 1 (<jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101). <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>n this study, these <jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101 tests were assessed using another sensitive method, that is, a luciferase immunoprecipitation system (<jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content>), to identify a better confirmatory test for <jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101 infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study Design and Methods<\/jats:title><jats:p>Plasma samples from 54 qualified donors and 114 <jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101 screening\u2013positive donors were tested by <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content> for antibodies against <jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101 <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ag, <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>ax, <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>nv, and <jats:styled-content style=\"fixed-case\">HBZ<\/jats:styled-content> recombinant proteins. The donors were categorized into six groups, namely, (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>) qualified donors, screening positive; (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">IF<\/jats:styled-content> positive; (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">IF<\/jats:styled-content> negative; (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> positive; (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>) <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> negative; and (<jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">VI<\/jats:styled-content>) screening positive in the previous blood donation, but <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content>\u2010indeterminate during this study period.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roups <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">IV<\/jats:styled-content>, all plasma samples tested positive by <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content> for antibodies against <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>ag and <jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>nv proteins. In <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">V<\/jats:styled-content>, all samples tested negative by <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content>, whereas some <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">III<\/jats:styled-content> samples reacted with single or double antigens in <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content>. In <jats:styled-content style=\"fixed-case\">G<\/jats:styled-content>roup <jats:styled-content style=\"fixed-case\">VI<\/jats:styled-content>, the <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content> test identified a donor with suspected <jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101 infection. The first case of a blood donor with plasma that reacted with <jats:styled-content style=\"fixed-case\">HBZ<\/jats:styled-content> was identified by <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Reevaluation of the current <jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101 screening method using the <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content> test showed that both confirmatory tests had similar sensitivity and specificity only when <jats:styled-content style=\"fixed-case\">WB<\/jats:styled-content> indeterminate results were eliminated. <jats:styled-content style=\"fixed-case\">LIPS<\/jats:styled-content> is a promising method for detecting and characterizing <jats:styled-content style=\"fixed-case\">HTLV<\/jats:styled-content>\u20101 antibodies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12911","type":"journal-article","created":{"date-parts":[[2014,11,3]],"date-time":"2014-11-03T04:07:39Z","timestamp":1414987659000},"page":"880-889","source":"Crossref","is-referenced-by-count":11,"title":["Reevaluation of confirmatory tests for human <scp>T<\/scp>\u2010cell leukemia virus <scp>T<\/scp>ype 1 using a luciferase immunoprecipitation system in blood donors"],"prefix":"10.1111","volume":"55","author":[{"given":"Rika A.","family":"Furuta","sequence":"first","affiliation":[{"name":"Japanese Red Cross, Kinki Block Blood Center  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyong","family":"Ma","sequence":"additional","affiliation":[{"name":"Laboratory of Virus Control Institute for Virus Research Kyoto University  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Matsuoka","sequence":"additional","affiliation":[{"name":"Laboratory of Virus 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