{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T15:00:28Z","timestamp":1787065228984,"version":"build-2736575974"},"reference-count":61,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T00:00:00Z","timestamp":1415836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000850","name":"National Heart and Lung Institute","doi-asserted-by":"publisher","award":["R01 HL095479"],"award-info":[{"award-number":["R01 HL095479"]}],"id":[{"id":"10.13039\/501100000850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,4]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Clinical and animal studies indicate that transfusions of older stored red blood cells (\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      s) impair clinical outcomes as compared to fresh\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      transfusions. It has been suggested that this effect is due to inhibition of nitric oxide (\n                      <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>\n                      )\u2010mediated vasodilation after transfusion of older\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      units. However, to date this effect has not been identified in human transfusion recipients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Study Design and Methods<\/jats:title>\n                    <jats:p>\n                      Forty\u2010three hospitalized patients with transfusion orders were randomly assigned to receive either fresh (&lt;14 days) or older stored (&gt;21 days)\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      units. Before transfusion, and at selected time points after the start of transfusion, endothelial function was assessed using noninvasive flow\u2010mediated dilation assays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After transfusion of older\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      units, there was a significant reduction in\n                      <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>\n                      \u2010mediated vasodilation at 24 hours after transfusion (p\u2009=\u20090.045), while fresh\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      transfusions had no effect (p\u2009=\u20090.231).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study suggests for the first time a significant inhibitory effect of transfused\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      units stored more than 21 days on\n                      <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>\n                      \u2010mediated vasodilation in anemic hospitalized patients. This finding lends further support to the hypothesis that deranged\n                      <jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>\n                      signaling mediates adverse clinical effects of older\n                      <jats:styled-content style=\"fixed-case\">RBC<\/jats:styled-content>\n                      transfusions. Future investigations will be necessary to address possible confounding factors and confirm these results.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.12919","type":"journal-article","created":{"date-parts":[[2014,11,13]],"date-time":"2014-11-13T03:34:53Z","timestamp":1415849693000},"page":"782-790","source":"Crossref","is-referenced-by-count":30,"title":["Effects of storage\u2010aged red blood cell transfusions on endothelial function in hospitalized patients"],"prefix":"10.1111","volume":"55","author":[{"given":"Robert","family":"Neuman","sequence":"first","affiliation":[{"name":"Division of Cardiology Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salim","family":"Hayek","sequence":"additional","affiliation":[{"name":"Division of Cardiology Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayaz","family":"Rahman","sequence":"additional","affiliation":[{"name":"Division of Cardiology Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph C.","family":"Poole","sequence":"additional","affiliation":[{"name":"Division of Cardiology Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivek","family":"Menon","sequence":"additional","affiliation":[{"name":"Division of Cardiology Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salman","family":"Sher","sequence":"additional","affiliation":[{"name":"Division of Cardiology Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James L.","family":"Newman","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Center for Transfusion and Cellular Therapies Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulaiman","family":"Karatela","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Center for Transfusion and Cellular Therapies Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Polhemus","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Louisiana State University Health Sciences Center  New Orleans Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Lefer","sequence":"additional","affiliation":[{"name":"Department of Pharmacology Louisiana State University Health Sciences Center  New Orleans Louisiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"De Staercke","sequence":"additional","affiliation":[{"name":"National Center on Birth Defects and Developmental Disabilities Division of Blood Disorders Centers for Disease Control and Prevention  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Hooper","sequence":"additional","affiliation":[{"name":"National Center on Birth Defects and Developmental Disabilities Division of Blood Disorders Centers for Disease Control and Prevention  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arshed A.","family":"Quyyumi","sequence":"additional","affiliation":[{"name":"Division of Cardiology Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Roback","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Center for Transfusion and Cellular Therapies Emory University School of Medicine  Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,11,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.158.22.2464"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.01026.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2006.00958.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa041065"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200605000-00005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.jp.7210566"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41060803.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.1997.01430300062013"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.CCM.0000165566.82925.14"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3171\/jns.2004.101.1.0001"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199902113400601"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa066240"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-007-0741-9"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707958104"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708160104"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V93.5.1770"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe078019"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1177\/108925320400800402"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa070403"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02583.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03466.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-11-539353"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-462945"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2010.05.014"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2011.03.001"},{"key":"e_1_2_8_27_1","unstructured":"KochCG.The Red Cell Storage Duration and Outcomes in Cardic Surgery study. Clinical Trialsgov.2007[cited 2012 Aug 17]. Available from:http:\/\/clinicaltrialsgov\/ct2\/show\/NCT00458783"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03656.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12361"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58753"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12264"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.008698"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03186.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e32833ec217"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-01-132092"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-10-245001"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12111"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20120675"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03748.x"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2011.1.475"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.106.083303"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.02044"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.110.150821"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2011.02.051"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1177\/1358863X13508446"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.04.040"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.67.060603.090918"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1089\/152308603768295230"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117852"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000025404.78001.D8"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03094.x"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.91.5.1314"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1097\/ALN.0b013e31825575e6"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03615.x"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.HYP.0000092882.65699.19"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e3182a74a9b"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacl.2011.08.006"},{"key":"e_1_2_8_58_1","first-page":"68","article-title":"Characterization of macro\u2010and microvascular function and structure in patients with type 2 diabetes mellitus","volume":"1","author":"Keymel S","year":"2011","journal-title":"Am J Cardiovasc Dis"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10554-010-9616-1"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-11-451229"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1989.tb06286.x"},{"key":"e_1_2_8_62_1","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1182\/blood.V63.4.803.803","article-title":"Inappropriately low red cell 2,3\u2010diphosphoglycerate and p50 in transfused beta\u2010thalassemia","volume":"63","author":"Correra A","year":"1984","journal-title":"Blood"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.12919","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.12919","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T07:48:09Z","timestamp":1695973689000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.12919"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,11,13]]},"references-count":61,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2015,4]]}},"alternative-id":["10.1111\/trf.12919"],"URL":"https:\/\/doi.org\/10.1111\/trf.12919","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,11,13]]}}}