{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:42:05Z","timestamp":1788345725688,"version":"build-2803163510"},"reference-count":56,"publisher":"Wiley","issue":"6pt2","license":[{"start":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T00:00:00Z","timestamp":1418601600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Canadian Blood Services (CBS) \/Canadian Institutes of Health Research (CIHR) Partnership","award":["FRN6897"],"award-info":[{"award-number":["FRN6897"]}]},{"name":"CBS Intramural Operating Grant program","award":["2013-IG-AL00545"],"award-info":[{"award-number":["2013-IG-AL00545"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,6]]},"abstract":"Background<\/jats:title>Several monoclonal antibodies to CD<\/jats:styled-content>44 can successfully ameliorate murine immune thrombocytopenia (ITP<\/jats:styled-content>). As these antibodies may be a potential replacement for intravenous immune globulin (IVIG<\/jats:styled-content>) in the treatment of ITP<\/jats:styled-content> and other autoimmune diseases, an understanding of their mechanisms of action is important. The role of the inhibitory F<\/jats:styled-content>c receptor (Fc\u03b3RIIb<\/jats:styled-content>) in the mechanism of action of IVIG<\/jats:styled-content> and therapeutic CD<\/jats:styled-content>44 antibodies remains uncertain. To assess if Fc\u03b3RIIb<\/jats:styled-content> in splenic macrophages plays a critical role in the action of these two therapeutics, splenectomized mice and mice genetically deficient in Fc\u03b3RIIb<\/jats:styled-content> on different backgrounds were evaluated.<\/jats:p><\/jats:sec>Study Design and Methods<\/jats:title>Thrombocytopenia was induced in Fc\u03b3RIIb<\/jats:styled-content>\u2010deficient mice on B<\/jats:styled-content>6;129S<\/jats:styled-content>, C57BL<\/jats:styled-content>\/6, and BALB<\/jats:styled-content>\/C<\/jats:styled-content> backgrounds, as well as splenectomized mice and control mice by platelet (PLT<\/jats:styled-content>) antibody. PLT<\/jats:styled-content> counts were enumerated before and after treatment with anti\u2010CD<\/jats:styled-content>44, red blood cell antibodies, or IVIG<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Anti\u2010CD<\/jats:styled-content>44 is ineffective at inhibiting thrombocytopenia in B<\/jats:styled-content>6;129S Fc\u03b3RIIb<\/jats:styled-content>\u2010deficient mice but, like IVIG<\/jats:styled-content>, is effective in splenectomized mice and Fc\u03b3RIIb<\/jats:styled-content>\u2010deficient mice on the BALB<\/jats:styled-content>\/C<\/jats:styled-content> and C57BL<\/jats:styled-content>\/6 background.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These data suggest that 1) the B<\/jats:styled-content>6;129S<\/jats:styled-content> background itself is unlikely to be the sole reason for anti\u2010CD<\/jats:styled-content>44's inability to function in B<\/jats:styled-content>6;129S Fc\u03b3RIIb<\/jats:styled-content>\u2010deficient mice, 2) the simple loss of macrophage Fc\u03b3RIIb<\/jats:styled-content> expression alone is insufficient to explain anti\u2010CD<\/jats:styled-content>44 ameliorative function, and 3) a combination of mouse background genes in addition to Fc\u03b3RIIb<\/jats:styled-content> genetic disruption may affect the ability of anti\u2010CD<\/jats:styled-content>44 to function therapeutically. Similarities between IVIG<\/jats:styled-content> and anti\u2010CD<\/jats:styled-content>44 mechanisms suggest that patients responsive to IVIG<\/jats:styled-content> may also potentially respond to anti\u2010CD<\/jats:styled-content>44 treatment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.12957","type":"journal-article","created":{"date-parts":[[2014,12,15]],"date-time":"2014-12-15T07:44:45Z","timestamp":1418629485000},"page":"1492-1500","source":"Crossref","is-referenced-by-count":12,"title":["CD<\/scp>44 antibody\u2013mediated amelioration of murine immune thrombocytopenia (ITP): mouse background determines the effect of Fc<\/scp>\u03b3RIIb genetic disruption"],"prefix":"10.1111","volume":"55","author":[{"given":"Andrew R.","family":"Crow","sequence":"first","affiliation":[{"name":"Canadian Blood Services Centre for Innovation Toronto Ontario Canada"},{"name":"Keenan Research Centre for Biomedical Science St Michael's Hospital Toronto Ontario Canada"},{"name":"Department of 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