{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:14:55Z","timestamp":1789877695860,"version":"4.0.1"},"reference-count":29,"publisher":"Wiley","issue":"6pt2","license":[{"start":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T00:00:00Z","timestamp":1429056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Fetal human platelet antigen (HPA) genotyping is required to determine whether the fetus is at risk and whether prenatal interventions to prevent fetal bleeding are required in pregnant women with a history of fetal and neonatal alloimmune thrombocytopenia (FNAIT). Methods for noninvasive genotyping of HPA alleles with the use of maternal plasma cell\u2010free DNA were published recently but do lack internal controls to exclude false\u2010negative results.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Cell\u2010free DNA was isolated from plasma of four pregnant women with a history of FNAIT caused by anti\u2010HPA\u20101a and controls. A primer panel was designed to target sequences flanking single\u2010nucleotide polymorphisms (SNPs)\/exonic regions of<jats:italic>ITGB3<\/jats:italic>(HPA\u20101),<jats:italic>ITGA2B<\/jats:italic>(HPA\u20103),<jats:italic>ITGA2<\/jats:italic>(HPA\u20105),<jats:italic>CD109<\/jats:italic>(HPA\u201015),<jats:italic>RHD<\/jats:italic>,<jats:italic>RHCE<\/jats:italic>,<jats:italic>KEL<\/jats:italic>,<jats:italic>DARC<\/jats:italic>,<jats:italic>SLC14A1<\/jats:italic>,<jats:italic>GYPA<\/jats:italic>,<jats:italic>GYPB<\/jats:italic>, and<jats:italic>SRY<\/jats:italic>. These regions and eight anonymous SNPs were massively parallel sequenced by semiconductor technology.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The mean (\u00b1SD) number of reads for targeted SNPs was 5255 (\u00b12838). Fetal DNA was detected at a median of 4.5 (range, 2\u20108) polymorphic loci. The mean fractional fetal DNA concentration in cell\u2010free maternal plasma was 8.36% (range, 4.79%\u201015.9%). For HPA\u20101, nonmaternal<jats:italic>ITGB3<\/jats:italic>sequences (c.176T, HPA\u20101a) were detected in all HPA\u20101ab fetuses. One HPA\u20101bb fetus was unequivocally identified, showing the pregnancy was not at risk of FNAIT.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>We have successfully established massively parallel sequencing as a novel reliable method for noninvasive genotyping of fetal HPA\u20101a alleles. This technique may also allow the safe detection of other fetal blood group polymorphisms frequently involved in FNAIT and hemolytic disease of the newborn.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13102","type":"journal-article","created":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T06:45:10Z","timestamp":1429080310000},"page":"1538-1544","source":"Crossref","is-referenced-by-count":44,"title":["Noninvasive fetal genotyping of human platelet antigen\u20101a using targeted massively parallel sequencing"],"prefix":"10.1111","volume":"55","author":[{"given":"Sandra","family":"Wienzek\u2010Lischka","sequence":"first","affiliation":[{"name":"Institute for Clinical Immunology and Transfusion Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Krautwurst","sequence":"additional","affiliation":[{"name":"Institute for Clinical Immunology and Transfusion 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