{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T11:07:50Z","timestamp":1791544070619,"version":"4.3.4"},"reference-count":21,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2015,8,11]],"date-time":"2015-08-11T00:00:00Z","timestamp":1439251200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Combined chemotherapy with carboplatin and paclitaxel is first\u2010line treatment for lung and ovarian cancer. Drug\u2010induced antibodies to carboplatin are rare but can cause severe, even fatal, hemolysis. Paclitaxel\u2010induced immune hemolysis has not been reported. We describe a case of immune\u2010mediated hemolysis associated with antibodies to carboplatin and paclitaxel in a woman with ovarian cancer who had received multiple chemotherapeutic agents over 7 years, including several courses of these two drugs. She required many transfusions. During a chemotherapy infusion the patient became hypotensive, was pale, and had rigors and red urine. The nadir hematocrit was 12.4%; peak bilirubin and lactate dehydrogenase were 16.3 mg\/dL and 1188 units\/L, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Blood samples collected within hours after chemotherapy and 2 days later were tested for antibodies to carboplatin and paclitaxel.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The direct antiglobulin test was positive with anti\u2010IgG (3+) and anti\u2010C3 (2+). The plasma collected shortly after chemotherapy agglutinated carboplatin\u2010treated red blood cells (RBCs); untreated and paclitaxel\u2010treated RBCs both reacted at the antiglobulin test most likely due to circulating carboplatin, paclitaxel, or both drugs. Serum collected 2 days later agglutinated (titer 2) and sensitized (titer 128) carboplatin\u2010treated RBCs; untreated and paclitaxel\u2010treated RBCs were nonreactive. An acid eluate reacted weakly in the presence of polyethylene glycol with carboplatin\u2010treated RBCs. The serum reacted with untreated and enzyme\u2010treated RBCs in the presence of soluble carboplatin and paclitaxel.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Anti\u2010carboplatin and the first example of anti\u2010paclitaxel were detected in this patient's sample.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13255","type":"journal-article","created":{"date-parts":[[2015,8,12]],"date-time":"2015-08-12T05:10:51Z","timestamp":1439356251000},"page":"2949-2954","source":"Crossref","is-referenced-by-count":14,"title":["Drug\u2010induced immune hemolytic anemia associated with anti\u2010carboplatin and the first example of anti\u2010paclitaxel"],"prefix":"10.1111","volume":"55","author":[{"given":"Regina M.","family":"Leger","sequence":"first","affiliation":[{"name":"American Red Cross Blood Services, Southern California Region Pomona California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shweta","family":"Jain","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest and the <sup>2<\/sup>Departments of Hematology and Laboratory Medicine, University of Washington Medical Center Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa A.","family":"Nester","sequence":"additional","affiliation":[{"name":"Bloodworks Northwest and the <sup>2<\/sup>Departments of Hematology and Laboratory Medicine, University of Washington Medical Center Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Kaplan","sequence":"additional","affiliation":[{"name":"Swedish Medical Center Seattle Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.21307\/immunohematology-2019-100"},{"key":"e_1_2_9_3_1","volume-title":"Immune hemolytic anemias","author":"Petz LD","year":"2004"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.02028.x"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.1998.00615.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.2146\/ajhp070432"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001813-199504000-00018"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1996.36111297091748.x"},{"key":"e_1_2_9_9_1","first-page":"173","article-title":"Carboplatin induced fatal autoimmune hemolytic anemia: first reported case","volume":"1","author":"Dacha S","year":"2010","journal-title":"World J Oncol"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12729"},{"key":"e_1_2_9_11_1","doi-asserted-by":"crossref","unstructured":"HaleyKM RussellTB BoshkovL et al.Fatal carboplatin\u2010induced immune hemolytic anemia in a child with a brain tumor.J Blood Med2014;5:55\u20108.","DOI":"10.2147\/JBM.S59192"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1998.38698326336.x"},{"key":"e_1_2_9_13_1","doi-asserted-by":"crossref","first-page":"85","DOI":"10.21307\/immunohematology-2019-102","article-title":"How we investigate drug\u2010induced immune hemolytic anemia","volume":"30","author":"Leger RM","year":"2014","journal-title":"Immunohematology"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03321.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.21307\/immunohematology-2019-335","article-title":"Warm autoantibody or drug\u2010dependent antibody? 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