{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T11:30:44Z","timestamp":1785583844947,"version":"3.56.0"},"reference-count":50,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2015,9,7]],"date-time":"2015-09-07T00:00:00Z","timestamp":1441584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Massive exchange transfusion of 42\u2010day\u2010old red blood cells (RBCs) in a canine model of <jats:italic>Staphylococcus aureus<\/jats:italic> pneumonia resulted in in vivo hemolysis with increases in cell\u2010free hemoglobin (CFH), transferrin\u2010bound iron (TBI), non\u2013transferrin\u2010bound iron (NTBI), and mortality. We have previously shown that washing 42\u2010day\u2010old RBCs before transfusion significantly decreased NTBI levels and mortality, but washing 7\u2010day\u2010old RBCs increased mortality and CFH levels. We now report the results of altering volume, washing, and age of RBCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Two\u2010year\u2010old purpose\u2010bred infected beagles were transfused with increasing volumes (5\u201010, 20\u201040, or 60\u201080 mL\/kg) of either 42\u2010 or 7\u2010day\u2010old RBCs (n\u2009=\u200936) or 80 mL\/kg of either unwashed or washed RBCs with increasing storage age (14, 21, 28, or 35 days; n\u2009=\u200940).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>All volumes transfused (5\u201080 mL\/kg) of 42\u2010day\u2010old RBCs resulted in alike (i.e., not significantly different) increases in TBI during transfusion as well as in CFH, lung injury, and mortality rates after transfusion. Transfusion of 80 mL\/kg RBCs stored for 14, 21, 28, and 35 days resulted in increased CFH and NTBI in between levels found at 7 and 42 days of storage. However, washing RBCs of intermediate ages (14\u201035 days) does not alter NTBI and CFH levels or mortality rates.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Preclinical data suggest that any volume of 42\u2010day\u2010old blood potentially increases risks during established infection. In contrast, even massive volumes of 7\u2010day\u2010old blood result in minimal CFH and NTBI levels and risks. In contrast to the extremes of storage, washing blood stored for intermediate ages does not alter risks of transfusion or NTBI and CFH clearance.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13275","type":"journal-article","created":{"date-parts":[[2015,9,7]],"date-time":"2015-09-07T10:17:34Z","timestamp":1441621054000},"page":"2564-2575","source":"Crossref","is-referenced-by-count":24,"title":["In a canine pneumonia model of exchange transfusion, altering the age but not the volume of older red blood cells markedly alters outcome"],"prefix":"10.1111","volume":"55","author":[{"given":"Irene","family":"Cort\u00e9s\u2010Puch","sequence":"first","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth E.","family":"Remy","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven B.","family":"Solomon","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junfeng","family":"Sun","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Wang","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariam","family":"Al\u2010Hamad","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth M.","family":"Kelly","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Derek","family":"Sinchar","sequence":"additional","affiliation":[{"name":"Vascular Medicine Institute University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Landon","family":"Bellavia","sequence":"additional","affiliation":[{"name":"Department of Physics and the Translational Science Center Wake Forest University Winston\u2010Salem North Carolina"},{"name":"Department of Physics University of Findlay Findlay Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamir","family":"Kanias","sequence":"additional","affiliation":[{"name":"Vascular Medicine Institute University of Pittsburgh Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Popovsky","sequence":"additional","affiliation":[{"name":"Haemonetics Corporation Braintree Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel B.","family":"Kim\u2010Shapiro","sequence":"additional","affiliation":[{"name":"Department of Physics and the Translational Science Center Wake Forest University Winston\u2010Salem North Carolina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harvey G.","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Natanson","sequence":"additional","affiliation":[{"name":"Critical Care Medicine Department Clinical Center, NIH Bethesda Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,7]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Standards for blood banks and transfusion services","year":"2011"},{"key":"e_1_2_8_3_1","unstructured":"WhitakerBI HenryRA.The 2011 national blood collection and utilization survey report [Internet]. Washington (DC): U.S.Department of Health & Human Services;2011 [cited 2015 Aug 20]. Available from:http:\/\/www.hhs.gov\/ash\/bloodsafety\/2011-nbcus.pdf"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/anae.12891"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03511.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708160104"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0b013e31828843e6"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.110.008698"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12130"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2012-10-462945"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-10-245001"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03197.x"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI59770"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e31828cf456"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12712"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa070403"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e318184aa11"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc10142"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/cc13045"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/lt.23695"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/TA.0b013e3181fa0019"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03466.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1414219"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1500704"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03521.x"},{"key":"e_1_2_8_26_1","first-page":"255","article-title":"A randomised controlled trial of standard transfusion versus fresher red blood cell use in intensive care (TRANSFUSE): protocol and statistical analysis plan","volume":"16","author":"Kaukonen KM","year":"2014","journal-title":"Crit Care Resusc"},{"key":"e_1_2_8_27_1","unstructured":"Washington University School of Medicine. Age of blood in children in pediatric intensive care units (ABC PICU). In:ClinicalTrials.gov[Internet]. Bethesda (MD): National Library of Medicine (US); 2013\u2010 [cited 2015 Aug 20]. Available from:https:\/\/clinicaltrials.gov\/ct2\/show\/NCT01977547. NLM Identifier: NCT01977547."},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-08-371849"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1213\/ANE.0b013e318241fcd5"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1097\/00000542-200605000-00005"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12607"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-11-539353"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2000.tb02249.x"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0195-5616(95)50153-6"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00589.2007"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2011.07.014"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.02850.x"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)01728-5"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.65.5.1944-1948.1997"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.1.29-37.2004"},{"key":"e_1_2_8_41_1","first-page":"507","article-title":"The effect of parenteral iron administration on the development of Staphylococcus aureus\u2010induced experimental pyelonephritis in rats","volume":"71","author":"Ang O","year":"1990","journal-title":"Int J Exp Pathol"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e3181e76274"},{"key":"e_1_2_8_43_1","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1542\/peds.60.6.908","article-title":"Increased incidence of gram\u2010negative neonatal sepsis with intramuscular iron administration","volume":"60","author":"Barry DM","year":"1977","journal-title":"Pediatrics"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2014.2726"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCM.0b013e3182741a54"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2013.12.014"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2008.07.013"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02583.x"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ahj.2009.12.045"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02661.x"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01642.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13275","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13275","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13275","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:23:39Z","timestamp":1695572619000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.13275"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,7]]},"references-count":50,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1111\/trf.13275"],"URL":"https:\/\/doi.org\/10.1111\/trf.13275","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,7]]}}}