{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T09:55:53Z","timestamp":1793354153561,"version":"4.5.0"},"reference-count":56,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,9,8]],"date-time":"2015-09-08T00:00:00Z","timestamp":1441670400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>To prevent the iatrogenic spread of variant Creutzfeldt\u2010Jakob disease (vCJD) between humans via blood products or transfusion, highly sensitive in vitro screening tests are necessary. Protein misfolding cyclic amplification (PMCA) is one such candidate test. However, plasma has been reported to inhibit the PMCA reaction. Therefore, we investigated the cell\u2010PMCA conditions that permit vCJD prion amplification in the presence of plasma.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Cell\u2010PMCA of vCJD samples was performed by adding various final concentrations of pooled plasma, citrate\u2010phosphate\u2010dextrose (CPD), albumin, globulin, or pooled plasma treated with ion exchangers. After heparin and plasma concentrations were optimized, multiround cell\u2010PMCA was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>When 1% to 50% of pooled plasma was added to heparinized cell\u2010PMCA, amplification efficiency showed a double\u2010peaked profile at less than 1% and 40% final plasma concentrations, indicating that plasma contains not only PMCA inhibitors but also promoters. Intravenous globulin did not inhibit cell\u2010PMCA, but the protein G\u2013bound fraction did. CPD, albumin\u2010depleted plasma, and the unbound fraction of anion\u2010exchange chromatography inhibited cell\u2010PMCA, but albumin and the unbound fraction of the cation\u2010exchange chromatography did not. The detection limit of abnormal prion protein in multiround cell\u2010PMCA, when maintaining the final plasma concentration at 40% at each round, was 10<jats:sup>\u221210<\/jats:sup> dilutions of a vCJD brain specimen.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>We have established a novel cell\u2010PMCA format in the presence of plasma without any pretreatment, where vCJD prion protein was amplified at comparable levels to that found without plasma. Our data suggest the feasibility of cell\u2010PMCA as a practical blood test for vCJD prions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13279","type":"journal-article","created":{"date-parts":[[2015,9,8]],"date-time":"2015-09-08T06:40:09Z","timestamp":1441694409000},"page":"223-230","source":"Crossref","is-referenced-by-count":8,"title":["Efficient propagation of variant <scp>C<\/scp>reutzfeldt\u2010<scp>J<\/scp>akob disease prion protein using the cell\u2010protein misfolding cyclic amplification technique with samples containing plasma and heparin"],"prefix":"10.1111","volume":"56","author":[{"given":"Masatoshi","family":"Oshita","sequence":"first","affiliation":[{"name":"Research and Development Division, Japan Blood Products Organization Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"Research and Development Division, Japan Blood Products Organization Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yumiko","family":"Takei","sequence":"additional","affiliation":[{"name":"Research and Development Division, Japan Blood Products Organization Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuko","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Neurological Science Tohoku University Graduate School of Medicine Sendai Miyagi Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Ironside","sequence":"additional","affiliation":[{"name":"National CJD Research and Surveillance Unit, Centre for Clinical Brain Sciences, University of Edinburgh, Western General Hospital Edinburgh United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuyuki","family":"Kitamoto","sequence":"additional","affiliation":[{"name":"Department of Neurological Science Tohoku University Graduate School of Medicine Sendai Miyagi Prefecture Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanori","family":"Morita","sequence":"additional","affiliation":[{"name":"Research and Development Division, Japan Blood Products Organization Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,9,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.23.13363"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)91412-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/383685a0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/39057"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)12075-5"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05411-3"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05403-4"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.f5675"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15486-X"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16811-6"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69835-8"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2516.2009.02181.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-04-152520"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03199.x"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0268-960X(98)90007-8"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62308-2"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth0308-211"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/35081095"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12037"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.2011.01525.x"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.4161\/pri.19430"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2294"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1315200"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.02.011"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2008.181"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(06)12001-7"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M603964200"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01979"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C110.113464"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.2008\/004226-0"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2006.11.056"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0013152"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2011.04.072"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi100370b"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0078710"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004202"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02595.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00078-11"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1286"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1465"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1812.120528"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja01207a034"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.07.016"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.04.040"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404650101"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.10.163600"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60057-3"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0054915"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23589"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2204"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2008.01954.x"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/2654"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.18996-0"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.4161\/pri.5.3.16893"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M114.569814"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/gzp031"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13279","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13279","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13279","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:46:06Z","timestamp":1695494766000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.13279"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,9,8]]},"references-count":56,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2016,1]]}},"alternative-id":["10.1111\/trf.13279"],"URL":"https:\/\/doi.org\/10.1111\/trf.13279","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,9,8]]}}}