{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T15:11:50Z","timestamp":1773414710547,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Human neutrophil antigen (HNA)\u22124a\/4bw is encoded by 230G&gt;A in <jats:italic>ITGAM<\/jats:italic>, which results in an Arg61His substitution of the \u03b1M chain (CD11b) of complement receptor 3 (CR3; CD11b\/18 or Mac\u20101). HNA\u20104a antibodies have been detected in the sera of female blood donors and in maternal sera that caused alloimmune neonatal neutropenia (ANN), in which maternal immunoglobulin (Ig)G antibodies against a paternally inherited HNA cross the placenta and destroy fetal and neonatal neutrophils. However, to date, antibodies specific for HNA\u20104b have not been reported. Here, we report the first two examples of HNA\u20104b antibodies.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>The two sera studied were both from previously pregnant females, one a multiparous female blood donor implicated in two separate transfusion reactions and the second a mother whose first pregnancy resulted in the birth of a severely neutropenic (0 \u00d7 10<jats:sup>6<\/jats:sup> neutrophils\/L) infant affected with ANN. Serum neutrophil antibody testing was by flow cytometry and CD11b\/18 monoclonal antibody immobilization of granulocyte antigens assay, and HNA genotyping was performed by polymerase chain reaction with sequence\u2010specific priming and allele\u2010specific 5\u2032 exonuclease assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Sera from both women contained IgG antibodies reactive only with HNA\u20104b+ neutrophils and both typed HNA\u20104a\/a. Both were immunized through pregnancy since their husbands and children all typed HNA\u20104a\/b.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The serologic results, together with the genotype results, confirm that these are the first reported cases of neutrophil antibodies specific for HNA\u20104b.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13287","type":"journal-article","created":{"date-parts":[[2015,9,2]],"date-time":"2015-09-02T19:26:38Z","timestamp":1441221998000},"page":"101-106","source":"Crossref","is-referenced-by-count":20,"title":["Two cases of maternal alloimmunization against human neutrophil alloantigen\u20104b, one causing severe alloimmune neonatal neutropenia"],"prefix":"10.1111","volume":"56","author":[{"given":"Brian R.","family":"Curtis","sequence":"first","affiliation":[{"name":"Platelet &amp; Neutrophil Immunology Laboratory BloodCenter of Wisconsin Milwaukee Wisconsin"},{"name":"Blood Research Institute BloodCenter of Wisconsin Milwaukee Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ashley S.","family":"Roman","sequence":"additional","affiliation":[{"name":"NYU Langone Medical Center New York New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mia J.","family":"Sullivan","sequence":"additional","affiliation":[{"name":"Platelet &amp; Neutrophil Immunology Laboratory BloodCenter of Wisconsin Milwaukee Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cindy S.","family":"Raven","sequence":"additional","affiliation":[{"name":"Michigan Blood Grand Rapids Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judy","family":"Larison","sequence":"additional","affiliation":[{"name":"Michigan Blood Grand Rapids Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee Ann","family":"Weitekamp","sequence":"additional","affiliation":[{"name":"Michigan Blood Grand Rapids Michigan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOH.0b013e32834babdd"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1986.tb04875.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/vox.12016"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02979.x"},{"key":"e_1_2_9_6_1","first-page":"154","article-title":"Leukocyte Beta2\u2010integrins; genes and disease","volume":"4","author":"MacPherson M","year":"2013","journal-title":"J Genet Syndr Gene Ther"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02913.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3148.2003.00413.x"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.00199.x"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03600.x"},{"key":"e_1_2_9_11_1","unstructured":"Fatalities reported to FDA following blood collection and transfusion: annual summary for fiscal year 2012 [Internet]. 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