{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T13:14:03Z","timestamp":1716038043759},"reference-count":15,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,1,22]],"date-time":"2016-01-22T00:00:00Z","timestamp":1453420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,4]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Beyond hemoglobin (Hb) levels and performance status, further surrogate markers of appropriate transfusion management should improve the quality of thalassemia care. We investigated the levels of peripheral circulating CD34+ stem cells as an independent marker of appropriate hematopoietic balance in patients with thalassemia.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Peripheral circulating CD34+ stem cells, colony\u2010forming unitgranulocyte, erythrocyte, macrophage, magakaryocyte (CF\u2010GEMM), colony\u2010forming unitgranulocyte\/macrophage (CFU\u2010GM), and erythroidburst\u2010forming units (BFU\u2010E) were assayed, according to standard procedures. Patients with thalassemia major (TM) and thalassemia intermedia (TI) were tested and compared to healthy controls. Demographic and clinical data were recorded.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Overall, 56 patients with TM (median age, 35 years; range, 13\u201052 years) and 13 with TI (median age, 44 years; range, 27\u201067 years) were evaluated. Annual red blood cell (RBC) transfusion requirements ranged from 10 to 65 units in all patients except four nontransfused cases. A significant increase in peripheral circulating stem cells was observed in patients, in comparison with healthy controls. Nontransfused patients showed the mean highest levels of stem cells (CD34, 32.5 \u00b1 14.8\/\u03bcL; BFU\u2010E, 41.3 \u00b1 22.8\/mL; CFU\u2010GM, 19.6 \u00b1 5.6\/mL; CFU\u2010GEMM, 9.0 \u00b1 6.1\/mL). CD34+ cell count was 6.9 \u00b1 4.5\/\u03bcL in TM (p = 0.014) and 11.8 \u00b1 14.8\/\u03bcL (p\u2009=\u20090.051) in TI. Furthermore, only in patients with TI was a significant increase in CFU\u2010GEMM (3.0 \u00b1 4.8 vs. 0.75 \u00b1 2.05\/mL, p\u2009=\u20090.0001) observed. At multivariate analysis, peripheral circulating CD34+ stem cells did not correlate with age, sex, smoking habit, number of RBCs units transfused, Hb levels, iron chelation therapy, history of splenectomy, and hypothyroidism.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Circulating peripheral CD34\u2009+\u2009stem cells are increased in \u03b2\u2010thalassemia, in particular in nontransfused patients, compared to healthy controls.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13452","type":"journal-article","created":{"date-parts":[[2016,1,23]],"date-time":"2016-01-23T04:18:51Z","timestamp":1453522731000},"page":"827-830","source":"Crossref","is-referenced-by-count":5,"title":["Hematopoietic peripheral circulating blood stem cells as an independent marker of good transfusion management in patients with \u03b2\u2010thalassemia: results from a preliminary study"],"prefix":"10.1111","volume":"56","author":[{"given":"Mariasanta","family":"Napolitano","sequence":"first","affiliation":[{"name":"UOC Ematologia con Trapianto, Universit\u00e0 di Palermo Palermo, Italy; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Calogera","family":"Gerardi","sequence":"additional","affiliation":[{"name":"Banca del Sangue da Cordone Ombelicale UOC Medicina Trasfusionale, PO \u201cGiovanni Paolo II,\u201d Sciacca Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Di Lucia","sequence":"additional","affiliation":[{"name":"Banca del Sangue da Cordone Ombelicale UOC Medicina Trasfusionale, PO \u201cGiovanni Paolo II,\u201d Sciacca Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pietro Andrea","family":"Accardo","sequence":"additional","affiliation":[{"name":"UOS Talassemia UOC Medicina Trasfusionale, PO \u201cGiovanni Paolo II,\u201d Sciacca Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luigi","family":"Rizzuto","sequence":"additional","affiliation":[{"name":"UOS Talassemia UOC Medicina Trasfusionale, PO \u201cGiovanni Paolo II,\u201d Sciacca Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Ferraro","sequence":"additional","affiliation":[{"name":"UOS Talassemia UOC Medicina Trasfusionale, PO \u201cGiovanni Paolo II,\u201d Sciacca Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sergio","family":"Siragusa","sequence":"additional","affiliation":[{"name":"UOC Ematologia con Trapianto, Universit\u00e0 di Palermo Palermo, Italy; and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippo","family":"Buscemi","sequence":"additional","affiliation":[{"name":"UOS Talassemia UOC Medicina Trasfusionale, PO \u201cGiovanni Paolo II,\u201d Sciacca Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,22]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199907083410207"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1980.tb07183.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000109141.48980.37"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00532.2009"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.12.024"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.00376.2002"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.5530160815"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/stem.5530100605"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190500144235"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2012.076240"},{"key":"e_1_2_8_12_1","volume-title":"Guidelines for the clinical management of thalassemia","author":"Cappellini MD","year":"2009"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0197-1859(97)81348-9"},{"key":"e_1_2_8_14_1","first-page":"178","article-title":"Different activation signals detected by fixation of stimulator cells","volume":"21","author":"Sutherland RM","year":"1989","journal-title":"Transplant Proc"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2011.195"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1995.tb08351.x"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13452","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13452","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13452","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T07:50:56Z","timestamp":1694850656000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.13452"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,22]]},"references-count":15,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2016,4]]}},"alternative-id":["10.1111\/trf.13452"],"URL":"https:\/\/doi.org\/10.1111\/trf.13452","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,22]]}}}