{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T23:09:04Z","timestamp":1789427344877,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T00:00:00Z","timestamp":1453075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["273985"],"award-info":[{"award-number":["273985"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Dengue virus (DENV) is a transfusion\u2010transmissible arbovirus that threatens blood donor systems with approximately 200 million high\u2010titer asymptomatic infections occurring annually. Here we investigated the viability of DENV during storage of donor\u2010derived platelet (PLT) and red blood cell (RBC) units. While purified PLTs have been shown to generate viable DENV, RBCs are replication incompetent. Combined with different storage criteria, distinct virus persistence profiles were anticipated in PLT and RBC units.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Mimicking the virus titer of asymptomatic donors, purified DENV was spiked (10<jats:sup>5<\/jats:sup>\u201010<jats:sup>6<\/jats:sup> infectious units\/mL) into PLT or RBC units produced and stored according to blood bank operating procedures. DENV was measured by infectious plaque\u2010forming assays and by quantitative reverse transcription\u2013polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In both PLT (7 days, 20\u201024\u00b0C) and RBC (42 days, 1\u20106\u00b0C) units, infectious DENV persisted throughout storage despite logarithmic decay. In buffer alone, DENV infectivity was insignificant by Day 1 at 20 to 24\u00b0C or 14 days at 1 to 6\u00b0C. Infectious virus production was identified in stored PLT units using a translation inhibitor and supported by virus genome replication. Surprisingly, DENV was also produced in RBC units, implying the involvement of cells other than RBCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Both virus propagation and effects independent of cell function mitigate the intrinsic lability of DENV. Nevertheless, the overall rapid storage decay suggests that aged PLT and RBC units may be safer. These data raise awareness to the possible persistence of other conceivably more robust RNA viruses during the storage of cellular blood products.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13454","type":"journal-article","created":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T15:30:59Z","timestamp":1453131059000},"page":"1129-1137","source":"Crossref","is-referenced-by-count":28,"title":["Dengue virus persists and replicates during storage of platelet and red blood cell units"],"prefix":"10.1111","volume":"56","author":[{"given":"Michael R.","family":"Sutherland","sequence":"first","affiliation":[{"name":"Centre for Innovation Canadian Blood Services Vancouver British Columbia Canada"},{"name":"Centre for Blood Research and Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayo Y.","family":"Simon","sequence":"additional","affiliation":[{"name":"Centre for Innovation Canadian Blood Services Vancouver British Columbia Canada"},{"name":"Centre for Blood Research and Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"},{"name":"African Centre of Excellence on Neglected Tropical Diseases and Forensic Biotechnology Ahmadu Bello University Zaria Nigeria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Serrano","sequence":"additional","affiliation":[{"name":"Centre for Innovation Canadian Blood Services Vancouver British Columbia Canada"},{"name":"Centre for Blood Research and Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Schubert","sequence":"additional","affiliation":[{"name":"Centre for Innovation Canadian Blood Services Vancouver British Columbia Canada"},{"name":"Centre for Blood Research and Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason P.","family":"Acker","sequence":"additional","affiliation":[{"name":"Network Centre for Applied Development Canadian Blood Services Vancouver British Columbia Canada"},{"name":"Department of Laboratory Medicine and Pathology University of Alberta Edmonton Alberta Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward L.G.","family":"Pryzdial","sequence":"additional","affiliation":[{"name":"Centre for Innovation Canadian Blood Services Vancouver British Columbia Canada"},{"name":"Centre for Blood Research and Department of Pathology and Laboratory Medicine University of British Columbia Vancouver British Columbia Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,18]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1110265"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-3527(08)60342-5"},{"key":"e_1_2_8_5_1","first-page":"299","article-title":"Epidemiology of dengue: past, present and future prospects","volume":"5","author":"Murray NE","year":"2013","journal-title":"Clin Epidemiol"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074582"},{"key":"e_1_2_8_7_1","article-title":"Fatal hemophagocytic lymphohistiocytosis associated with locally acquired dengue virus infection \u2010 New Mexico and Texas, 2012","volume":"63","author":"Sharp TM","year":"2014","journal-title":"MMWR Morb Mortal Wkly 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