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We isolated<jats:italic>Bartonella henselae<\/jats:italic>from 1.2% of 500 studied blood donors and demonstrated that the bacteria remain viable in red blood cell units after 35 days of experimental infection. Now, we aim to evaluate the possibility of<jats:italic>B. henselae<\/jats:italic>transmission by blood transfusion in a mouse model.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Eight BALB\/c mice were intraperitoneal inoculated with a 30 \u00b5L of suspension with 10<jats:sup>4<\/jats:sup>CFU\/mL of<jats:italic>B. henselae<\/jats:italic>and a second group of eight mice were inoculated with saline solution and used as control. After 96 hours of inoculation, the animals were euthanized. We collected blood and tissue samples from skin, liver, and spleen. Thirty microliters of blood from four<jats:italic>Bartonella<\/jats:italic>\u2010inoculated animals were transfused into a new group (n\u2009=\u20094). Another group received blood from the control animals.<jats:italic>B. henselae<\/jats:italic>infection was investigated by conventional and nested polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Blood samples from all 24 mice were negative by molecular tests though half of the tissue samples were positive by nested PCR in the intraperitoneal<jats:italic>Bartonella<\/jats:italic>\u2010investigated animals. Tissues from two of the four mice that received blood transfusions from<jats:italic>Bartonella<\/jats:italic>\u2010inoculated animals were also nested PCR positives.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Transmission of<jats:italic>B. henselae<\/jats:italic>by transfusion is possible in mice even when donor animals have undetectable bloodstream infection. The impact of human<jats:italic>Bartonella<\/jats:italic>sp. transmission through blood transfusion recipients must be evaluated.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13545","type":"journal-article","created":{"date-parts":[[2016,3,12]],"date-time":"2016-03-12T07:26:12Z","timestamp":1457767572000},"page":"1556-1559","source":"Crossref","is-referenced-by-count":21,"title":["<i>Bartonella henselae<\/i>transmission by blood transfusion in mice"],"prefix":"10.1111","volume":"56","author":[{"given":"Marilene Neves","family":"Silva","sequence":"first","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gislaine","family":"Vieira\u2010Damiani","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"},{"name":"Federal Institute of Education, Science and Technology Jaguariaiva PR Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marna Elise","family":"Ericson","sequence":"additional","affiliation":[{"name":"Department of Dermatology University of Minnesota Medical School Minneapolis Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kalpna","family":"Gupta","sequence":"additional","affiliation":[{"name":"Division of Hematology Oncology and Transplantation Department of Medicine University of Minnesota Medical School Minneapolis Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rovilson","family":"Gilioli","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Quality Control Multidisciplinary Center of Biological Investigation (CEMIB), University of Campinas (UNICAMP) Campinas SP Brazil."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amanda Roberta","family":"de Almeida","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina Rovani","family":"Drummond","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruno Grosselli","family":"Lania","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karina","family":"de Almeida Lins","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T\u00e2nia Cristina Benetti","family":"Soares","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Eduardo Neves Ferreira","family":"Velho","sequence":"additional","affiliation":[{"name":"Division of Dermatology Department of Medicine University of Campinas (UNICAMP) Medical School Campinas SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,10]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0003467"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.2.203"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02598-13"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-9571(96)00025-2"},{"key":"e_1_2_7_6_1","volume-title":"Bartonellosis o Enfermedad de Carrion. 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