{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T15:42:40Z","timestamp":1762875760752},"reference-count":9,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,4,4]],"date-time":"2016-04-04T00:00:00Z","timestamp":1459728000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The Lu<jats:sup>b<\/jats:sup> antigen is expressed on red blood cells (RBCs) of the majority of individuals in all populations. Its absence in transfused patients may lead to alloantibody production and mild\u2010to\u2010moderate transfusion reactions, and in pregnancies to mild hemolytic disease of the fetus and newborn. This report describes the results of discrepancy resolution between apparent <jats:italic>LU*A\/LU*B<\/jats:italic> or <jats:italic>LU*B\/LU*B<\/jats:italic> genotypes and apparent Lu(b\u2013) or Lu(b+ weak) phenotypes in one Asian and 10 Caucasian blood donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Whole blood samples were analyzed by molecular methods to resolve discrepancies between Lu(b\u2013) phenotypes detected by serology and Lu(b+) phenotypes predicted by genotyping. RBC agglutination assays were performed with commercial and patient antisera by tube or gel column methods. Genotyping was performed on commercial arrays that target the <jats:italic>LU*A\/LU*B<\/jats:italic> polymorphism at Position c.230. The discrepancies were resolved by sequencing of genomic DNA and in some cases by sequencing of cloned DNA fragments.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Eleven new alleles with coding sequence variants were identified, seven in the <jats:italic>KLF1<\/jats:italic> gene, which are presumed to act dominantly to silence <jats:italic>LU<\/jats:italic> expression, and four in the <jats:italic>LU<\/jats:italic> gene itself. The alleles are <jats:italic>KLF1*114delC<\/jats:italic>, <jats:italic>KLF1*298T<\/jats:italic>, <jats:italic>KLF1*304C<\/jats:italic>,<jats:italic>484insC<\/jats:italic>, <jats:italic>KLF1*304C,1000del2<\/jats:italic>, <jats:italic>KLF1*621G<\/jats:italic>, <jats:italic>KLF1*948delC<\/jats:italic>, <jats:italic>KLF1*1040A,1045delT<\/jats:italic>, <jats:italic>LU*B(559T,711T,714T)<\/jats:italic>, <jats:italic>LU*B(611A,638T)<\/jats:italic>, <jats:italic>LU*B(1049del2ins3),<\/jats:italic> and <jats:italic>LU*B(1306T,1340T,1671T,1742T)<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Besides confirming common phenotypes and detecting rare antigen\u2010negative phenotypes, the use of molecular methods in blood donor typing can prompt the identification of new alleles through discrepancy resolution.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13556","type":"journal-article","created":{"date-parts":[[2016,4,4]],"date-time":"2016-04-04T15:39:06Z","timestamp":1459784346000},"page":"1413-1418","source":"Crossref","is-referenced-by-count":5,"title":["Identification of new <i>KLF1<\/i> and <i>LU<\/i> alleles during the resolution of Lutheran typing discrepancies"],"prefix":"10.1111","volume":"56","author":[{"given":"Felix","family":"Garcia\u2010Sanchez","sequence":"first","affiliation":[{"name":"Centro de Transfusion de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Pardi","sequence":"additional","affiliation":[{"name":"Clinical Immunology and Transfusion Medicine Sahlgrenska University Hospital Gothenburg Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawinee","family":"Kupatawintu","sequence":"additional","affiliation":[{"name":"National Blood Centre, Thai Red Cross Society Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicole","family":"Thornton","sequence":"additional","affiliation":[{"name":"International Blood Group Reference Laboratory, NHS Blood and Transplant Bristol UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel\u2010Angel","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Centro de Transfusion de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Irene","family":"Lucea","sequence":"additional","affiliation":[{"name":"Centro de Transfusion de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chhavi","family":"Sood","sequence":"additional","affiliation":[{"name":"Progenika Biopharma\u2013Grifols Medford Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gorka","family":"Ochoa\u2010Garay","sequence":"additional","affiliation":[{"name":"Grifols Shared Services San Marcos Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,4]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-145672"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12184"},{"key":"e_1_2_7_4_1","first-page":"54","volume-title":"Applied blood group serology","author":"Issit PD.","year":"1985"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2005.04369.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1982.tb00015.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.12.4608"},{"key":"e_1_2_7_8_1","first-page":"S107","article-title":"Novel mutations in EKLF\/KLF1 encoding In(Lu) phenotype","volume":"50","author":"Crowley J","year":"2010","journal-title":"Transfusion"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22218"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781118493595"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13556","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13556","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,18]],"date-time":"2023-09-18T11:35:55Z","timestamp":1695036955000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.13556"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,4,4]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1111\/trf.13556"],"URL":"https:\/\/doi.org\/10.1111\/trf.13556","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,4,4]]}}}