{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T17:46:25Z","timestamp":1775065585101,"version":"3.50.1"},"reference-count":12,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,3,20]],"date-time":"2016-03-20T00:00:00Z","timestamp":1458432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>A patient with B+ sickle cell disease received 3 units of red blood cells (RBCs) from two O+ donors and developed fever and hypotension after the first unit, consistent with an acute transfusion reaction (ATR). Anti\u2010B titers in plasma from each O+ donor were markedly elevated and nondiscriminatory. In order to evaluate the potential for the transfused units to produce complement\u2010mediated hemolysis of B+ RBCs, hemolytic complement testing was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Plasma from each donor was diluted in veronal buffer and incubated with B+ RBCs, and free hemoglobin was measured by spectrophotometer in the complement hemolysis using human erythrocytes (CHUHE) assay. Peptide inhibitor of complement C1 (PIC1) was used to confirm antibody\u2010initiated complement pathway activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>A 96\u2010fold difference (p\u2009=\u20090.014) in hemolysis was measured between plasma samples from the two O+ donors using the CHUHE assay. The extremely high degree of hemolysis produced by the one plasma was inhibited by PIC1 in a dose\u2010dependent manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results indicate that hemolytic complement testing with the CHUHE assay can be used to assess the risk of antibody\u2010initiated, complement\u2010mediated hemolysis from a transfusion beyond what can be achieved with antibody titers alone.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13592","type":"journal-article","created":{"date-parts":[[2016,3,21]],"date-time":"2016-03-21T03:08:43Z","timestamp":1458529723000},"page":"1845-1848","source":"Crossref","is-referenced-by-count":11,"title":["Discriminating complement\u2010mediated acute transfusion reaction for type O+ red blood cells transfused into a B+ recipient with the complement hemolysis using human erythrocytes (CHUHE) assay"],"prefix":"10.1111","volume":"56","author":[{"given":"Kenji M.","family":"Cunnion","sequence":"first","affiliation":[{"name":"Department of Pediatrics Eastern Virginia Medical School"},{"name":"Children's Specialty Group"},{"name":"Children's Hospital of the King's Daughters"},{"name":"Department of Microbiology and Molecular Cell Biology Eastern Virginia Medical School"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela S.","family":"Hair","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Eastern Virginia Medical School"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neel K.","family":"Krishna","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Eastern Virginia Medical School"},{"name":"Department of Microbiology and Molecular Cell Biology Eastern Virginia Medical School"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela H.","family":"Whitley","sequence":"additional","affiliation":[{"name":"American Red Cross Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Corinne L.","family":"Goldberg","sequence":"additional","affiliation":[{"name":"American Red Cross Blood Services Durham North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel A.","family":"Fadeyi","sequence":"additional","affiliation":[{"name":"Department of Pathology Wake Forest School of Medicine Winston\u2010Salem North Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lanne Y.","family":"Maes","sequence":"additional","affiliation":[{"name":"American Red Cross Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,3,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/9781444303513.ch52"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3148.1992.tb00128.x"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2015.01.003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/307093"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1309\/VXY1ULAFUY6E6JT3"},{"key":"e_1_2_7_7_1","first-page":"493","volume-title":"AABB technical manual","author":"Downes KA","year":"2011"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1423-0410.1998.tb05433.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1067\/mob.2001.112970"},{"key":"e_1_2_7_10_1","first-page":"421","volume-title":"AABB technical manual","author":"Walker PS","year":"2014"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.168.1.127"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2012.07.012"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132446"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13592","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13592","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T10:50:05Z","timestamp":1696157405000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.13592"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,20]]},"references-count":12,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1111\/trf.13592"],"URL":"https:\/\/doi.org\/10.1111\/trf.13592","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,20]]}}}