{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T04:42:22Z","timestamp":1779165742567,"version":"3.51.4"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,5,3]],"date-time":"2016-05-03T00:00:00Z","timestamp":1462233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Preparation of umbilical cord blood units (CBUs) for infusion requires a step of dilution or washing to reduce the toxicity of the dimethyl sulfoxide present in the freezing solution. However, the worldwide shortage of clinical\u2010grade dextran 40, the most widely used cord blood dilution or washing solution, prompted us to search for an alternative solution. We elected to evaluate the performance of alternative solutions that could be used as potential replacements for the dextran 40\u2013based solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Frozen CBUs were rapidly thawed and immediately diluted 1:4 in each of 10 dilution solution variants of dextran 40, Plasma\u2010lyte, or Hespan. We compared these alternatives by assessing viability, CD34, CD45, and colony\u2010forming units recovery postthaw.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>A significantly lower CD34 and CD45 recovery was observed in all solutions without human serum albumin (HSA). All solutions with 5% HSA gave recovery, viability, and potency figures similar to the dextran 40\/HSA solution.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Based on our results and considering that Plasma\u2010lyte A (PA)\/HSA is already used for the thawing of CD34 from mobilized blood, we conclude that PA\/HSA could be a safe and efficient solution for the replacement of dextran 40\u2013based dilution solutions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13633","type":"journal-article","created":{"date-parts":[[2016,5,4]],"date-time":"2016-05-04T16:19:21Z","timestamp":1462378761000},"page":"1786-1791","source":"Crossref","is-referenced-by-count":10,"title":["An alternative to dextran for the thawing of cord blood units"],"prefix":"10.1111","volume":"56","author":[{"given":"Marc","family":"Cloutier","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology and Bio\u2010Informatics Laval University Qu\u00e9bec City Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carl","family":"Simard","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Bio\u2010Informatics Laval University Qu\u00e9bec City Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Jobin","sequence":"additional","affiliation":[{"name":"<sup>1<\/sup>Department of Research and Development H\u00e9ma\u2010Qu\u00e9bec Qu\u00e9bec City Qu\u00e9bec, Canada"},{"name":"Department of Biochemistry, Microbiology and Bio\u2010Informatics Laval University Qu\u00e9bec City Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diane","family":"Fournier","sequence":"additional","affiliation":[{"name":"Public Cord Blood Bank H\u00e9ma\u2010Qu\u00e9bec Saint\u2010Laurent Qu\u00e9bec Canada."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"N\u00e9ron","sequence":"additional","affiliation":[{"name":"<sup>1<\/sup>Department of Research and Development H\u00e9ma\u2010Qu\u00e9bec Qu\u00e9bec City Qu\u00e9bec, Canada"},{"name":"Department of Biochemistry, Microbiology and Bio\u2010Informatics Laval University Qu\u00e9bec City Qu\u00e9bec"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,3]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70127-3"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2006.11.005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-01-0294"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01327.x"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02803.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.22.10119"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2009.08.009"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13015"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2015.08.007"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12122"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704030"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/term.109"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0320(19980415)34:2<61::AID-CYTO1>3.0.CO;2-F"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2015.08.016"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/bmt.2013.152"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12428"},{"key":"e_1_2_8_18_1","unstructured":"FDA safety communication: boxed warning on increased mortality and severe renal injury and additional warning on risk of bleeding for use of hydroxyethyl starch solutions in some settings [Internet]. 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