{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T15:53:02Z","timestamp":1791215582996,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p><jats:italic>Babesia microti<\/jats:italic> is a protozoan parasite responsible for the majority of reported cases of human babesiosis and a major risk to the blood supply. Laboratory screening of blood donors may help prevent transfusion\u2010transmitted babesiosis but there is no Food and Drug Administration\u2013approved screening method yet available. Development of a sensitive, specific, and highly automated <jats:italic>B. microti<\/jats:italic> antibody assay for diagnosis of acute babesiosis and blood screening could have an important impact on decreasing the health burden of <jats:italic>B. microti<\/jats:italic> infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Herein, we take advantage of recent advances in <jats:italic>B. microti<\/jats:italic> genomic analyses, field surveys of the reservoir host, and human studies in endemic areas to apply a targeted immunomic approach to the discovery of <jats:italic>B. microti<\/jats:italic> antigens that serve as signatures of active or past babesiosis infections. Of 19 glycosylphosphatidylinositol (GPI)\u2010anchored protein candidates (BmGPI1\u201019) identified in the <jats:italic>B. microti<\/jats:italic> proteome, 17 were successfully expressed, printed on a microarray chip, and used to screen sera from uninfected and <jats:italic>B. microti<\/jats:italic>\u2013infected mice and humans to determine immune responses that are associated with active and past infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Antibody responses to various <jats:italic>B. microti<\/jats:italic> BmGPI antigens were detected and BmGPI12 was identified as the best biomarker of infection that provided high sensitivity and specificity when used in a microarray antibody assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>BmGPI12 alone or in combination with other BmGPI proteins is a promising candidate biomarker for detection of <jats:italic>B. microti<\/jats:italic> antibodies that might be useful in blood screening to prevent transfusion\u2010transmitted babesiosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13640","type":"journal-article","created":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T07:04:53Z","timestamp":1463468693000},"page":"2085-2099","source":"Crossref","is-referenced-by-count":38,"title":["A targeted immunomic approach identifies diagnostic antigens in the human pathogen <i>Babesia microti<\/i>"],"prefix":"10.1111","volume":"56","author":[{"given":"Emmanuel","family":"Cornillot","sequence":"first","affiliation":[{"name":"Institut de Biologie Computationnelle (IBC), Institut de Recherche en Canc\u00e9rologie de Montpellier (IRCM\u2010INSERM U1194), Institut r\u00e9gional du Cancer Montpellier (ICM) and Universit\u00e9 de Montpellier Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amina","family":"Dassouli","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Section of Infectious Diseases, Yale School 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