{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T19:55:45Z","timestamp":1772135745329,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T00:00:00Z","timestamp":1463443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Blood transfusion safety is based on reliable donor screening for transmissible infections such as the hepatitis C virus (HCV) infection.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>A novel HCV core\u2013specific antibody was assayed on random single donations from 2007 first\u2010time blood donors who tested negative for anti\u2010HCV and HCV RNA on routine screening. Sample collection broke the code between donations and donors for ethical reasons.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Forty\u2010two donations (2.1%) displayed reactivity in the novel test. The specificity of the reactivity was evaluated by a peptide inhibition assay, and testing against additional nonoverlapping HCV core peptide epitopes and other HCV antigens was performed on these samples. Six donations (14.3%; 0.30% from the total) were considered to contain anti\u2010HCV after such supplemental testing. HCV RNA detection was also performed in peripheral blood mononuclear cells (PBMNCs) and serum or plasma samples from reactive donors after virus concentration by ultracentrifugation. HCV RNA tested negative in all PBMNCs samples, and a very low amount of viral genome was detected in serum or plasma concentrates from three anti\u2010HCV core\u2013reactive donors (7.1%) but not among concentrates from 100 randomly selected nonreactive donors. Sequencing of these polymerase chain reaction products revealed differences between the isolates that excluded partially sample contamination from a common source.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These findings argue in favor of an ongoing occult HCV infection among these blood donors and account for some rather low, but perhaps not negligible, infection risk for such donations. Future studies involving larger samples of donations from traceable donors would enlighten the significance of these findings for the viral safety of the blood supply.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13645","type":"journal-article","created":{"date-parts":[[2016,5,17]],"date-time":"2016-05-17T07:04:08Z","timestamp":1463468648000},"page":"1883-1890","source":"Crossref","is-referenced-by-count":13,"title":["Detection of hepatitis C virus (HCV) core\u2013specific antibody suggests occult HCV infection among blood donors"],"prefix":"10.1111","volume":"56","author":[{"given":"Juan A.","family":"Quiroga","sequence":"first","affiliation":[{"name":"Fundaci\u00f3n para el Estudio de las Hepatitis Virales Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Avell\u00f3n","sequence":"additional","affiliation":[{"name":"Department of Virology Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Bartolom\u00e9","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n para el Estudio de las Hepatitis Virales Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Andr\u00e9u","sequence":"additional","affiliation":[{"name":"Centro de Transfusi\u00f3n de la Cruz Roja Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elena","family":"Flores","sequence":"additional","affiliation":[{"name":"Centro de Transfusi\u00f3n de la Comunidad de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda I.","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Centro de Hemoterapia y Hemodonaci\u00f3n de Castilla y Le\u00f3n Valladolid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roc\u00edo","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Centro de Transfusi\u00f3n de la Cruz Roja Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sonia","family":"P\u00e9rez","sequence":"additional","affiliation":[{"name":"Centro de Hemoterapia y Hemodonaci\u00f3n de Castilla y Le\u00f3n Valladolid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A.","family":"Richart","sequence":"additional","affiliation":[{"name":"Centro de Transfusi\u00f3n de la Comunidad de Madrid Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inmaculada","family":"Castillo","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n para el Estudio de las Hepatitis Virales Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javier","family":"Alcover","sequence":"additional","affiliation":[{"name":"Laboratorios DIATER S.A Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Palacios","sequence":"additional","affiliation":[{"name":"Laboratorios DIATER S.A Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicente","family":"Carre\u00f1o","sequence":"additional","affiliation":[{"name":"Fundaci\u00f3n para el Estudio de las Hepatitis Virales Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 M.","family":"Echevarr\u00eda","sequence":"additional","affiliation":[{"name":"Department of Virology Centro Nacional de Microbiolog\u00eda, Instituto de Salud Carlos III Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22759"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis368"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040085"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.02622.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13024"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1086\/380202"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02750-06"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2008030293"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008128"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2008.08.021"},{"key":"e_1_2_9_12_1","first-page":"131A","article-title":"A new high sensitivity anti\u2010HCV Core kit allows detecting hepatitis C virus infected individuals not detected by other available anti\u2010HCV assays [abstract]","volume":"56","author":"Quiroga JA","year":"2012","journal-title":"Hepatology"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01339-14"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/339237a0"},{"key":"e_1_2_9_15_1","first-page":"448","article-title":"Spanish group for the study of blood donors with risk of HCV transmission","volume":"17","author":"Le\u00f3n P","year":"1999","journal-title":"Enferm Infecc Microbiol Clin"},{"key":"e_1_2_9_16_1","unstructured":"Strategic plan for the management of hepatitis C in the National Health System [Internet]. Madrid: Ministerio de Sanidad Servicios Sociales e Igualdad de Espa\u00f1a.2015[cited 2015 Nov 6]. Available from:http:\/\/www.msssi.gob.es\/ciudadanos\/enfLesiones\/enfTransmisibles\/docs\/plan_estrategico_hepatitis_C.pdf."},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24219"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03524.x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1994.34294143940.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.1998.7510032.x"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-81-2-461"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890430409"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.10186"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01012-07"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1157\/13083370"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.11.4942"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0140312"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.10.048"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3408"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI73104"},{"key":"e_1_2_9_31_1","first-page":"15","article-title":"The hidden epidemic of hepatitis C virus infection in the United States: occult transmission and burden of disease","volume":"21","author":"Ward JW.","year":"2013","journal-title":"Topics Antiviral Med"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13645","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13645","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/trf.13645","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T10:48:31Z","timestamp":1696157311000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.13645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,5,17]]},"references-count":30,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2016,7]]}},"alternative-id":["10.1111\/trf.13645"],"URL":"https:\/\/doi.org\/10.1111\/trf.13645","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,5,17]]}}}