{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T11:09:24Z","timestamp":1772881764167,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,5,27]],"date-time":"2016-05-27T00:00:00Z","timestamp":1464307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004726","name":"Ministry of Health","doi-asserted-by":"publisher","award":["RF 2010"],"award-info":[{"award-number":["RF 2010"]}],"id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004726","name":"Ministry of Health","doi-asserted-by":"publisher","award":["141\/RF\u20102010\u20102303934"],"award-info":[{"award-number":["141\/RF\u20102010\u20102303934"]}],"id":[{"id":"10.13039\/501100004726","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Low\u2010risk myelodysplastic syndromes (MDS) show several immunologic abnormalities, including increased frequency of autoimmune manifestations and\/or overt autoimmune diseases, whose prognostic significance still remains controversial.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>We studied the presence of erythroblast antibodies in mitogen\u2010stimulated bone marrow (BM) cultures of 70 patients with early\u2010stage MDS (refractory anemia and refractory anemia with ringed sideroblasts).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Sixty\u2010six percent of patients showed positive erythroblast antibodies, along with BM erythroid hyperplasia and a hemolytic picture in the peripheral blood. Supernatants from positive cultures induced an increase of overall cellularity, the appearance of erythroblastic clustering, and dyserythropoietic signs in normal BM. We identified CD45<jats:sup>dim<\/jats:sup>Gly\u2010A<jats:sup>dim<\/jats:sup>CD71<jats:sup>bright<\/jats:sup> cells (red blood cell precursors at different maturation stage) as the target of the antibodies. Erythropoietin (EPO) levels were reduced and EPO receptors (EPO\u2010R) increased in BM culture supernatants from positive patients. However, flow cytometric analysis showed that neither EPO nor EPO\u2010R was involved in an abnormal stimulation driven by these autoantibodies. Values of the proapoptotic protein Bax were increased in positive patients and Bcl\u20102 levels were decreased, although not significantly.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>MDS patients with anti\u2010erythroblast autoimmunity showed increased BM apoptosis, suggesting that the autoimmune reaction may contribute to an unfavorable BM microenvironment for optimal erythropoiesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13652","type":"journal-article","created":{"date-parts":[[2016,5,27]],"date-time":"2016-05-27T13:42:58Z","timestamp":1464356578000},"page":"2037-2041","source":"Crossref","is-referenced-by-count":15,"title":["Detection of erythroblast antibodies in mitogen\u2010stimulated bone marrow cultures from patients with myelodysplastic syndromes"],"prefix":"10.1111","volume":"56","author":[{"given":"Anna","family":"Zaninoni","sequence":"first","affiliation":[{"name":"U.O. Oncoematologia and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca G.","family":"Imperiali","sequence":"additional","affiliation":[{"name":"U.O. Oncoematologia and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Cattaneo","sequence":"additional","affiliation":[{"name":"Servizio di Citofluorimetria, U.O. Laboratorio Centrale di Analisi Chimico Cliniche e Microbiologiche Dipartimento dei Servizi Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Soverini","sequence":"additional","affiliation":[{"name":"U.O. Oncoematologia and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesca","family":"Binda","sequence":"additional","affiliation":[{"name":"U.O. Oncoematologia and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Porretti","sequence":"additional","affiliation":[{"name":"Servizio di Citofluorimetria, U.O. Laboratorio Centrale di Analisi Chimico Cliniche e Microbiologiche Dipartimento dei Servizi Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agostino","family":"Cortelezzi","sequence":"additional","affiliation":[{"name":"U.O. Oncoematologia and Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico Milano"},{"name":"Universit\u00e0 Degli Studi di Milano Milano Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wilma","family":"Barcellini","sequence":"additional","affiliation":[{"name":"U.O. 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