{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:51:49Z","timestamp":1787064709283,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T00:00:00Z","timestamp":1465516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Virginia Innovation Partnership U.S. Department of Commerce i6 Challenge grant"},{"name":"Children's Health Foundation of The Children's Hospital of the King's Daughters"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Acute hemolytic transfusion reactions have a broad clinical presentation from mild and transitory signs and symptoms to shock, disseminated intravascular coagulation, renal failure, and death. We have recently developed a rat model of acute intravascular hemolysis showing that the classical complement pathway mediates antibody\u2010dependent hemolysis. The objective of this study was to evaluate the role of the classical pathway inhibitor peptide inhibitor of complement C1 (PIC1) in this animal model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>STUDY DESIGN AND METHODS<\/jats:title>\n                    <jats:p>Male Wistar rats received a 15% transfusion of human red blood cells (RBCs) and blood was isolated from the animals up to 120 minutes. Animals received PIC1 either 2 minutes before or 0.5 minutes after transfusion. Sham\u2010, vehicle\u2010, and cobra venom factor (CVF)\u2010treated animals were used as control groups with a subset of rats also receiving an equivalent dose of intravenous immunoglobulin (IVIG) before transfusion. Blood was analyzed for transfused RBC survival by flow cytometry and free hemoglobin (Hb) in isolated plasma by spectrophotometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>Vehicle\u2010treated rats showed decreased human RBC survival and increased free Hb as expected. Rats receiving PIC1 before transfusion showed increased human RBC survival and decreased Hb similar to CVF\u2010treated rats. Notably, rats receiving PIC1 after initiation of transfusion showed similar decreases in hemolysis as animals receiving PIC1 before transfusion. Compared to IVIG and saline controls, PIC1\u2010treated animals demonstrated decreased hemolysis and protection from acute kidney injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>These results demonstrate that PIC1 has efficacy in an animal model of acute intravascular hemolysis in both prevention and rescue scenarios.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/trf.13674","type":"journal-article","created":{"date-parts":[[2016,6,10]],"date-time":"2016-06-10T06:41:14Z","timestamp":1465540874000},"page":"2133-2145","source":"Crossref","is-referenced-by-count":15,"title":["Peptide inhibitor of complement C1 modulates acute intravascular hemolysis of mismatched red blood cells in rats"],"prefix":"10.1111","volume":"56","author":[{"given":"Parvathi S.","family":"Kumar","sequence":"first","affiliation":[{"name":"Department of Pediatrics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haree K.","family":"Pallera","sequence":"additional","affiliation":[{"name":"Department of Pediatrics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela S.","family":"Hair","sequence":"additional","affiliation":[{"name":"Department of Pediatrics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magdielis Gregory","family":"Rivera","sequence":"additional","affiliation":[{"name":"Department of Pediatrics"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tushar A.","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Pediatrics"},{"name":"Department of Microbiology and Molecular Cell Biology"},{"name":"Children's Hospital of the King's Daughters"},{"name":"Children's Specialty Group Norfolk Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice L.","family":"Werner","sequence":"additional","affiliation":[{"name":"Department of Pediatrics"},{"name":"Children's Hospital of the King's Daughters"},{"name":"Children's Specialty Group Norfolk Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank A.","family":"Lattanzio","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences Eastern Virginia Medical School"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji M.","family":"Cunnion","sequence":"additional","affiliation":[{"name":"Department of Pediatrics"},{"name":"Department of Microbiology and Molecular Cell Biology"},{"name":"Children's Hospital of the King's Daughters"},{"name":"Children's Specialty Group Norfolk Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neel K.","family":"Krishna","sequence":"additional","affiliation":[{"name":"Department of Pediatrics"},{"name":"Department of Microbiology and Molecular Cell Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,10]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"WhitakerBI.The 2011 national blood collection and utilization survey report. 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Available from:http:\/\/www.redcrossblood.org\/learn-about-blood\/blood-facts-and-statistics."},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.f4303"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-pathol-012414-040318"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpa.2012.12.006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00007.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.emc.2014.04.012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/307093"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12882"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13144"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12876"},{"key":"e_1_2_7_13_1","first-page":"281","volume-title":"Samter's immunologic disease","author":"Frank MM","year":"2001"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1248943"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00406"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2010.07.012"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2012.07.012"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0132446"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12695"},{"key":"e_1_2_7_20_1","first-page":"216","article-title":"Characterization of the natural hemagglutinins in normal rat serum associated with a negative phase following tumor implantation","volume":"16","author":"Aptekman PM","year":"1956","journal-title":"Cancer Res"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.11613\/BM.2011.015"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.11.6796-6803.2001"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/762051"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1159\/000154811"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/PEC.0b013e31822c9b4c"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.114.1.297"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.arcped.2014.02.005"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13119"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13674","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.13674","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.13674","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,26]],"date-time":"2023-09-26T03:56:45Z","timestamp":1695700605000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.13674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,6,10]]},"references-count":27,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,8]]}},"alternative-id":["10.1111\/trf.13674"],"URL":"https:\/\/doi.org\/10.1111\/trf.13674","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,6,10]]}}}