{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T01:19:36Z","timestamp":1791422376100,"version":"4.3.3"},"reference-count":37,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,6,27]],"date-time":"2016-06-27T00:00:00Z","timestamp":1466985600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Frankfurter Stiftung f\u00fcr krebskranke Kinder"},{"name":"Hilfe f\u00fcr krebskranke Kinder Frankfurt e.V."},{"name":"Alfred und Angelika Gutermuth-Stiftung,"},{"name":"LOEWE Center for Cell and Gene Therapy Frankfurt"},{"DOI":"10.13039\/501100003495","name":"Hessisches Ministerium f\u00fcr Wissenschaft und Kunst (HMWK)","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003495","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Excessive T\u2010cell depletion (TCD) is a prerequisite for graft manufacturing in haploidentical stem cell (SC) transplantation by using either CD34 selection or direct TCD such as CD3\/CD19 depletion.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>To optimize graft composition we compared 1) direct or indirect TCD only, 2) a combination of CD3\/CD19\u2010depleted with CD34\u2010selected grafts, or 3) TCD twice for depletion improvement based on our 10\u2010year experience with 320 separations in graft manufacturing and quality control.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>SC recovery was significantly higher (85%, n\u2009=\u2009187 vs. 73%, n\u2009=\u2009115; p\u2009&lt;\u20090.0001), but TCD was inferior (median log depletion, \u22123.6 vs. \u22125.2) for CD3\/CD19 depletion compared to CD34 selection, respectively. For end products with less than \u22122.5 log TCD, a second depletion step led to a successful improvement in TCD. Thawing of grafts showed a high viability and recovery of SCs, but low NK\u2010cell yield. To optimize individualized graft engineering, a calculator was developed to estimate the results of the final graft based on the content of CD34+ and CD3+ cells in the leukapheresis product.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Finally, calculated splitting of the starting product followed by CD3\/19 depletion together with CD34+ graft manipulation may enable the composition of optimized grafts with high CD34+\u2010cell and minimal T\u2010cell content.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13694","type":"journal-article","created":{"date-parts":[[2016,6,27]],"date-time":"2016-06-27T12:26:32Z","timestamp":1467030392000},"page":"2336-2345","source":"Crossref","is-referenced-by-count":11,"title":["Optimization of individualized graft composition: CD3\/CD19 depletion combined with CD34 selection for haploidentical transplantation"],"prefix":"10.1111","volume":"56","author":[{"given":"Sabine","family":"Huenecke","sequence":"first","affiliation":[{"name":"Clinic for Pediatric and Adolescent Medicine University Hospital Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Bremm","sequence":"additional","affiliation":[{"name":"Clinic for Pediatric and Adolescent Medicine University Hospital Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Cappel","sequence":"additional","affiliation":[{"name":"Clinic for Pediatric and Adolescent Medicine University Hospital Frankfurt Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Esser","sequence":"additional","affiliation":[{"name":"GMP Development Unit Institute of Cellular Therapeutics, IFB\u2010TX, Hannover Medical School Hannover 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