{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:57:00Z","timestamp":1790060220024,"version":"4.0.1"},"reference-count":47,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,7,8]],"date-time":"2016-07-08T00:00:00Z","timestamp":1467936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Blood donors whose red blood cells (RBCs) exhibit a partial RhD phenotype, lacking some D epitopes, present as D+ in routine screening. Such phenotypes can exhibit low\u2010frequency antigens (LFAs) of clinical significance. The aim of this study was to describe the serologic and genetic profile for a blood donor with an apparent D+ phenotype carrying a variant <jats:italic>RHD<\/jats:italic> gene where D Exons 5 and 6 are replaced by <jats:italic>RHCE<\/jats:italic> Exon (5\u20106).<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Anti\u2010D monoclonal antibodies were used to characterize the presentation of RhD epitopes on the RBCs. <jats:italic>RHD<\/jats:italic> exon scanning and DNA sequencing of short\u2010 and long\u2010range polymerase chain reaction amplicons were used to determine the <jats:italic>RHD<\/jats:italic> structure and sequence. Extended phenotyping for LFAs RH23 (D<jats:sup>W<\/jats:sup>) and Rh32 was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The donor serology profile was consistent with partial RhD epitope presentation. The donor was hemizygous for an <jats:italic>RHD<\/jats:italic> variant allele described as <jats:italic>RHD*D\u2010CE(5\u20106)\u2010D<\/jats:italic> hybrid. The <jats:italic>RHCE<\/jats:italic> gene insert is at least 3.868 kb with 5\u2032 and 3\u2032 breakpoints between IVS4\u2009+\u2009132\u2013c.667 and IVS6\u2009+\u20091960\u2013IVS6\u2009+\u20092099, respectively. The sequence for this hybrid was assigned GenBank Accession Number KT099190.2. The RBCs were RH23 (D<jats:sup>W<\/jats:sup>)+ and Rh32\u2013.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>A novel <jats:italic>RHD*D\u2010CE(5\u20106)\u2010D<\/jats:italic> hybrid allele encodes a partial RhD epitope and carries the LFA RH23 (D<jats:sup>W<\/jats:sup>). This and the epitope profile resemble the partial DVa phenotype. Given that RBCs from this individual lack some RhD epitopes, there is an alloimmunization risk if the donor is exposed to D+ RBCs. Conversely, transfusions of RH23 (D<jats:sup>W<\/jats:sup>)+ cells to RH23 (D<jats:sup>W<\/jats:sup>)\u2013 recipients also pose an alloimmunization risk.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13713","type":"journal-article","created":{"date-parts":[[2016,7,8]],"date-time":"2016-07-08T12:34:34Z","timestamp":1467981274000},"page":"2322-2330","source":"Crossref","is-referenced-by-count":9,"title":["A D+ blood donor with a novel <i>RHD*D\u2010CE(5\u20106)\u2010D<\/i> gene variant exhibits the low\u2010frequency antigen RH23 (D<sup>W<\/sup>) characteristic of the partial DVa phenotype"],"prefix":"10.1111","volume":"56","author":[{"given":"Genghis H.","family":"Lopez","sequence":"first","affiliation":[{"name":"Clinical Services and Research Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunike C.","family":"McGowan","sequence":"additional","affiliation":[{"name":"Clinical Services and Research Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kelli A.","family":"McGrath","sequence":"additional","affiliation":[{"name":"Red Cell Reference Laboratory Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Abaca\u2010Cleopas","sequence":"additional","affiliation":[{"name":"Red Cell Reference Laboratory Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizna M.","family":"Schoeman","sequence":"additional","affiliation":[{"name":"Clinical Services and Research Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenda M.","family":"Millard","sequence":"additional","affiliation":[{"name":"Clinical Services and Research Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"O'Brien","sequence":"additional","affiliation":[{"name":"Clinical Services and Research Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yew\u2010Wah","family":"Liew","sequence":"additional","affiliation":[{"name":"Red Cell Reference Laboratory Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L.","family":"Flower","sequence":"additional","affiliation":[{"name":"Clinical Services and Research Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine A.","family":"Hyland","sequence":"additional","affiliation":[{"name":"Clinical Services and Research Australian Red Cross Blood Service Kelvin Grove Queensland Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,8]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"International Society of Blood Transfusion (ISBT). 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