{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T05:05:16Z","timestamp":1785474316061,"version":"3.56.0"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,8,2]],"date-time":"2016-08-02T00:00:00Z","timestamp":1470096000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"2013 Fiocruz-Pasteur"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,10]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Recent studies conducted in developed countries described hepatitis E virus (HEV) as an emerging infectious threat to blood safety. However, data on HEV among blood donors from southeast Asia are lacking.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Between July and August 2014, we assessed the presence of HEV immunoglobulin (Ig)G and IgM in 301 Cambodian blood donors. All samples were further tested for the presence of HEV RNA using an in\u2010house reverse transcription\u2013polymerase chain reaction. ORF2\/ORF3 phylogenetic analysis was performed on positive HEV RNA specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>We found HEV IgG in 28.2% of blood donors from Cambodia. Three blood donors tested positive for HEV IgM with three distinct patterns: IgM(+)\/IgG(\u2013)\/RNA(\u2013) (n\u00a0=\u00a01), IgM(+)\/IgG(+)\/RNA(\u2013) (n\u00a0=\u00a01), and IgM(+)\/IgG(+)\/RNA(+) (n\u00a0=\u00a01). Thus, the prevalence rates of HEV IgM and HEV RNA were 1.0 and 0.3%. Interestingly, the viremic blood donor harbored a HEV strain that belonged to Genotype 3 (HEV\u20103) and clustered with a Cambodian riverine HEV\u20103 isolate.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Due to the high frequency of Cambodian blood donors with positive HEV IgG, we conclude that HEV is endemic in this country. Large\u2010scale studies must be considered to determine whether Cambodian blood donation screening is warranted to enhance blood safety in regard to HEV. In addition, our findings suggest that river water may be a significant source of exposure to HEV\u20103.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13731","type":"journal-article","created":{"date-parts":[[2016,8,3]],"date-time":"2016-08-03T13:24:19Z","timestamp":1470230659000},"page":"2597-2601","source":"Crossref","is-referenced-by-count":19,"title":["Hepatitis E virus antibody prevalence, RNA frequency, and genotype among blood donors in Cambodia (Southeast Asia)"],"prefix":"10.1111","volume":"56","author":[{"given":"Janin","family":"Nouhin","sequence":"first","affiliation":[{"name":"HIV\/Hepatitis Unit Pasteur Institute in Cambodia Phnom Penh Cambodia"},{"name":"Universit\u00e9 Paris Diderot\u2010Paris 7"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophearot","family":"Prak","sequence":"additional","affiliation":[{"name":"HIV\/Hepatitis Unit Pasteur Institute in Cambodia Phnom Penh Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoann","family":"Madec","sequence":"additional","affiliation":[{"name":"Unit\u00e9 d'Epid\u00e9miologie des Maladies Emergentes, Institut Pasteur Paris France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Barennes","sequence":"additional","affiliation":[{"name":"Epidemiology and Public Health Unit Pasteur Institute in Cambodia Phnom Penh Cambodia"},{"name":"Epidemiologie\u2010Biostatistique ISPED, Centre INSERM U897 Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Weissel","sequence":"additional","affiliation":[{"name":"National Blood Transfusion Center Phnom Penh Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimcheng","family":"Hok","sequence":"additional","affiliation":[{"name":"National Blood Transfusion Center Phnom Penh Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Pavio","sequence":"additional","affiliation":[{"name":"UMR 1161 Virologie Anses Laboratoire de Sant\u00e9 Animale Maisons\u2010Alfort France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Rouet","sequence":"additional","affiliation":[{"name":"HIV\/Hepatitis Unit Pasteur Institute in Cambodia Phnom Penh Cambodia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,2]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000393"},{"key":"e_1_2_8_3_1","first-page":"185","article-title":"Hepatitis E vaccine: WHO position paper, May 2015","volume":"90","year":"2015","journal-title":"Wkly Epidemiol 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