{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:59:09Z","timestamp":1773010749739,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,9,9]],"date-time":"2016-09-09T00:00:00Z","timestamp":1473379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004467","name":"Program for Innovative Research Team of Ningbo Novel Photoelectric Materials and Devices","doi-asserted-by":"publisher","award":["Z99 CL999999"],"award-info":[{"award-number":["Z99 CL999999"]}],"id":[{"id":"10.13039\/501100004467","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Scianna (SC) blood group system comprises two antithetical antigens, Sc1 and Sc2, and five additional antigens. The antigens reside on a glycoprotein encoded by the erythroblast membrane\u2013associated protein (<jats:italic>ERMAP<\/jats:italic>) gene. For the common <jats:italic>ERMAP<\/jats:italic> alleles, we determined the full\u2010length nucleotide sequence that encodes the Scianna glycoprotein.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Blood donor samples from five populations were analyzed including 20 African Americans, 10 Caucasians, 10 Thai, five Asians, and five Hispanics for a total of 100 chromosomes. An assay was devised to determine the genomic sequence of the <jats:italic>ERMAP<\/jats:italic> gene in one amplicon, spanning 21.4 kb and covering Exons 2 to 12 and the intervening sequence (IVS). All alleles (confirmed haplotypes) were resolved without ambiguity.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Among 50 blood donors, we found 80 single\u2010nucleotide polymorphisms (SNPs), including six novel SNPs, in 21,308 nucleotides covering the coding sequence of the <jats:italic>ERMAP<\/jats:italic> gene and including the introns. The noncoding sequences harbored 75 SNPs (68 in the introns and seven in the 3\u2032\u2010UTR). No SNP indicative of a nonfunctional allele was detected. The nucleotide sequences for 48 <jats:italic>ERMAP<\/jats:italic> alleles (confirmed haplotypes) were determined by allele\u2010specific polymerase chain reaction and sequencing in 100 chromosomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>We documented 48 <jats:italic>ERMAP<\/jats:italic> alleles of 21,308 nucleotides each. The two nucleotide sequences available in GenBank for <jats:italic>ERMAP<\/jats:italic> alleles of similar length have not been found in our 100 chromosomes. Alleles determined without ambiguity can be used as templates to analyze next generation sequencing data, which will enhance the reliability in clinical diagnostics.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13801","type":"journal-article","created":{"date-parts":[[2016,9,9]],"date-time":"2016-09-09T15:11:18Z","timestamp":1473433878000},"page":"3047-3054","source":"Crossref","is-referenced-by-count":10,"title":["Full\u2010length nucleotide sequence of <i>ERMAP<\/i> alleles encoding Scianna (SC) antigens"],"prefix":"10.1111","volume":"56","author":[{"given":"Kshitij","family":"Srivastava","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eunah","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric","family":"Owens","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pairaya","family":"Rujirojindakul","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Medicine Prince of Songkla University Songkhla Thailand."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1631-7198","authenticated-orcid":false,"given":"Willy A.","family":"Flegel","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, National Institutes of Health Bethesda 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