{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,14]],"date-time":"2024-07-14T16:09:47Z","timestamp":1720973387067},"reference-count":20,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,10,23]],"date-time":"2016-10-23T00:00:00Z","timestamp":1477180800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Health and Labour Sciences Research Grant","award":["H26-IyakuA-Ippan-002"],"award-info":[{"award-number":["H26-IyakuA-Ippan-002"]}]},{"name":"Blood Safety Program against Transfusion-Transmitted Infections"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Dengue fever is caused by four related RNA viruses of the genus <jats:italic>Flavivirus<\/jats:italic>, dengue virus (DENV)\u20101, \u20102, \u20103, and \u20104, which are transmitted to humans by mosquitoes. Although DENV is not endemic in Japan, an autochthonous dengue outbreak occurred in 2014. Several transfusion\u2010transmitted cases have also been reported after the use of blood and plasma products in DENV\u2010endemic countries. The aim of this study was to develop a novel multiplex reverse transcription\u2013polymerase chain reaction (RT\u2010PCR) assay for DENV blood screening.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Large\u2010scale oligonucleotide screening was performed to obtain DENV\u2010specific primers and probes using a variety of DENV clinical isolates. A multiplex RT\u2010PCR assay was then developed using the identified oligonucleotides and the ability of this assay to detect DENV RNA was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>A number of oligonucleotides suitable for DENV RNA detection were identified and a novel DENV serotype\u2010specific multiplex RT\u2010PCR assay was successfully established. Comparative analysis revealed that the multiplex assay could detect levels of viral contamination as low as 100 viral copies\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This established serotype\u2010specific multiplex RT\u2010PCR assay provides a simple, sensitive, and quantitative detection method for DENV, which could be applied in the screening of blood samples to prevent transfusion\u2010transmitted DENV infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13875","type":"journal-article","created":{"date-parts":[[2016,10,24]],"date-time":"2016-10-24T04:14:16Z","timestamp":1477282456000},"page":"3094-3100","source":"Crossref","is-referenced-by-count":6,"title":["Development of a novel dengue virus serotype\u2010specific multiplex real\u2010time reverse transcription\u2013polymerase chain reaction assay for blood 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