{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:15:43Z","timestamp":1762899343556},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T00:00:00Z","timestamp":1482192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Virginia Blood Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The agglutination\u2010based cross\u2010matching method is sensitive for antibody binding to red blood cells but is only partially predictive of complement\u2010mediated hemolysis, which is important in many acute hemolytic transfusion reactions. Here, we describe complement hemolysis using human erythrocytes (CHUHE) assays that directly evaluate complement\u2010mediated hemolysis between individual serum\u2010plasma and red blood cell combinations. The CHUHE assay is used to evaluate correlations between agglutination titers and complement\u2010mediated hemolysis as well as the hemolytic potential of plasma from type A blood donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Plasma or serum from each type A blood donor was incubated with AB or B red blood cells in the CHUHE assay and measured for free hemoglobin release.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>CHUHE assays for serum or plasma demonstrate a wide, dynamic range and high sensitivity for complement\u2010mediated hemolysis for individual serum\/plasma and red blood cell combinations. CHUHE results suggest that agglutination assays alone are only moderately predictive of complement\u2010mediated hemolysis. CHUHE results also suggest that plasma from particular type A blood donors produce minimal complement\u2010mediated hemolysis, whereas plasma from other type A blood donors produce moderate to high\u2010level complement\u2010mediated hemolysis, depending on the red blood cell donor.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>The current results indicate that the CHUHE assay can be used to assess complement\u2010mediated hemolysis for plasma or serum from a type A blood donor, providing additional risk discrimination over agglutination titers alone.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.13948","type":"journal-article","created":{"date-parts":[[2016,12,21]],"date-time":"2016-12-21T03:42:43Z","timestamp":1482291763000},"page":"517-524","source":"Crossref","is-referenced-by-count":16,"title":["Discriminating the hemolytic risk of blood type A plasmas using the complement hemolysis using human erythrocytes (CHUHE) assay"],"prefix":"10.1111","volume":"57","author":[{"given":"Kenji M.","family":"Cunnion","sequence":"first","affiliation":[{"name":"Department of Pediatrics Eastern Virginia Medical School Norfolk Virginia"},{"name":"Children's Specialty Group Norfolk Virginia"},{"name":"Department of Pediatrics Children's Hospital of the King's Daughters Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela S.","family":"Hair","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neel K.","family":"Krishna","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Eastern Virginia Medical School Norfolk Virginia"},{"name":"Department of Microbiology and Molecular Cell Biology Eastern Virginia Medical School Norfolk Virginia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Megan 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