{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T13:54:10Z","timestamp":1760709250173},"reference-count":33,"publisher":"Wiley","issue":"3pt2","license":[{"start":{"date-parts":[[2017,2,5]],"date-time":"2017-02-05T00:00:00Z","timestamp":1486252800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004829","name":"Department of Science and Technology of Sichuan Province","doi-asserted-by":"publisher","award":["2014SZ0239"],"award-info":[{"award-number":["2014SZ0239"]}],"id":[{"id":"10.13039\/501100004829","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The real infection status of hepatitis B virus (HBV) of hepatitis\u00a0B surface antigen (HBsAg)\u2010negative yet nucleic acid test (NAT)\u2010positive blood donors is difficult to clarify. Detailed follow\u2010up study is needed for analyzing the infectivity of these blood donors.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Blood donors who screened negative for HBsAg and reactive for simultaneous NAT of HBV, hepatitis\u00a0C virus (HCV), and human immunodeficiency virus (HIV) were included in a follow\u2010up epidemiologic questionnaire survey and contributed follow\u2010up samples for further testing. The follow\u2010up samples were tested repeatedly for the serologic markers and HBV DNA. The genotypes and sequence mutations of HBV infected by 11 HBV DNA\u2013positive donors were analyzed through the amplification and sequencing of HBV S region.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Of the 46 donors included in this study, 89.1% were infected with HBV (41\/46), including one (2.2%) window period infection, three (6.5%) recovered infections, and 37 (80.4%) occult HBV infections (OBIs). The S region of HBV was successfully amplified and sequenced for seven donors, five infected with Genotype B (71.4%), one with Genotype C (14.3%), and one with Genotype D (14.3%). Mutations in the S region were detected in four donors (57.1%)<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>This is the first detailed study with multiple follow\u2010up testing of the HBV infection status among blood donors who were tested negative for HBsAg and reactive for simultaneous NAT of HBV, HCV, and HIV. Most of these donors were infected with HBV with very low viral load. Our findings indicate that it is important to improve the sensitivity of NAT so as to decrease the residual risk of transfusion\u2010transmitted HBV infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14014","type":"journal-article","created":{"date-parts":[[2017,2,6]],"date-time":"2017-02-06T03:17:44Z","timestamp":1486351064000},"page":"832-840","source":"Crossref","is-referenced-by-count":2,"title":["The characteristics of hepatitis\u00a0B surface antigen (HBsAg)\u2010negative hepatitis\u00a0B virus (HBV) infection in Chinese blood donors: a follow\u2010up study of donors tested negative for HBsAg and reactive for simultaneous nucleic acid testing of HBV, hepatitis\u00a0C virus, and human immunodeficiency virus"],"prefix":"10.1111","volume":"57","author":[{"given":"Zhaofu","family":"Guo","sequence":"first","affiliation":[{"name":"Yunnan Dehong Blood Center Luxi City Dehong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Fu","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences Chengdu Sichuan China."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yijin","family":"Yin","sequence":"additional","affiliation":[{"name":"Yunnan Dehong Blood Center Luxi City Dehong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Funeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Yunnan Dehong Blood Center Luxi City Dehong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiqing","family":"Yin","sequence":"additional","affiliation":[{"name":"Yunnan Dehong Blood Center Luxi City Dehong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingxing","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Blood Transfusion, Chinese Academy of Medical Sciences 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