{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T12:16:18Z","timestamp":1789906578792,"version":"4.0.1"},"reference-count":52,"publisher":"Wiley","issue":"3pt2","license":[{"start":{"date-parts":[[2017,2,14]],"date-time":"2017-02-14T00:00:00Z","timestamp":1487030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Zika virus (ZIKV) has spread rapidly in the Pacific and throughout the Americas and is associated with severe congenital and adult neurologic outcomes. Nucleic acid amplification technology (NAT) assays were developed for diagnostic applications and for blood donor screening on high\u2010throughput NAT systems. We distributed blinded panels to compare the analytical performance of blood screening relative to diagnostic NAT assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>A 25\u2010member, coded panel (11 half\u2010log dilutions of a 2013 French Polynesia ZIKV isolate and 2015 Brazilian donor plasma implicated in transfusion transmission, and 3 negative controls) was sent to 11 laboratories that performed 17 assays with 2 to 12 replicates per panel member. Results were analyzed for the percentage reactivity at each dilution and by probit analysis to estimate the 50% and 95% limits of detection (LOD<jats:sub>50<\/jats:sub>and LOD<jats:sub>95<\/jats:sub>, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Donor\u2010screening NAT assays that process approximately 500 \u00b5L of plasma into amplification reactions were comparable in sensitivity (LOD<jats:sub>50<\/jats:sub>and LOD<jats:sub>95<\/jats:sub>, 2.5 and 15\u201018 copies\/mL) and were approximately 10\u2010fold to 100\u2010fold more sensitive than research laboratory\u2010developed and diagnostic reverse transcriptase\u2010polymerase chain reaction tests that process from 10 to 30 \u00b5L of plasma per amplification. Increasing sample input volume assayed with the Centers for Disease Control and Prevention reverse transcriptase\u2010polymerase chain reaction assays increased the LODs by 10\u2010fold to 30\u2010fold.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Blood donor\u2010screening ZIKV NAT assays demonstrate similar excellent sensitivities to assays currently used for screening for transfusion\u2010transmitted viruses and are substantially more sensitive than most other laboratory\u2010developed and diagnostic ZIKV reverse transcriptase\u2010polymerase chain reaction assays. Enhancing sensitivities of laboratory\u2010developed and diagnostic assays may be achievable by increasing sample input.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14031","type":"journal-article","created":{"date-parts":[[2017,2,14]],"date-time":"2017-02-14T06:06:52Z","timestamp":1487052412000},"page":"734-747","source":"Crossref","is-referenced-by-count":32,"title":["Relative analytical sensitivity of donor nucleic acid amplification technology screening and diagnostic real\u2010time polymerase chain reaction assays for detection of Zika virus RNA"],"prefix":"10.1111","volume":"57","author":[{"given":"Mars","family":"Stone","sequence":"first","affiliation":[{"name":"Blood Systems Research Institute University of California\u2010San Francisco San Francisco California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion C.","family":"Lanteri","sequence":"additional","affiliation":[{"name":"Blood Systems Research Institute University of California\u2010San Francisco 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