{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T12:58:05Z","timestamp":1771333085186,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T00:00:00Z","timestamp":1487203200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000014","name":"Canadian Blood Services","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000014","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Contamination of platelet concentrates (PCs) with <jats:italic>Staphylococcus aureus<\/jats:italic> is one of the most significant ongoing transfusion safety risks in developed countries.<\/jats:p><\/jats:sec><jats:sec><jats:title>CASE REPORT<\/jats:title><jats:p>This report describes a transfusion reaction in an elderly patient diagnosed with acute myeloid leukemia, transfused with a 4\u2010day\u2010old buffy coat PC through a central venous catheter. The transfusion was interrupted when a large fibrous clot in the PC obstructed infusion pump flow. Shortly afterward, a red blood cell (RBC) unit transfusion started. After septic symptoms were developed, the RBC transfusion was also interrupted. While the RBC unit tested negative for bacterial contamination, the PC and the patient samples were found to be contaminated with a <jats:italic>S.\u00a0aureus<\/jats:italic> strain that exhibited the same phenotypic and genome sequencing profiles. The isolated <jats:italic>S.\u00a0aureus<\/jats:italic> forms biofilms and produces the superantigen enterotoxin\u2010like U, which was detected in a sample of the transfused PCs. The patient received posttransfusion antibiotic treatment and had her original central line removed and replaced.<\/jats:p><\/jats:sec><jats:sec><jats:title>DISCUSSION<\/jats:title><jats:p>As the implicated PC had been tested for bacterial contamination during routine screening yielding negative results, this is a false\u2010negative transfusion sepsis case. Using a point\u2010of\u2010care test could have prevented the transfusion reaction. This report highlights the increasing incidence of <jats:italic>S.\u00a0aureus<\/jats:italic> as a major PC contaminant with grave clinical implications. Importantly, <jats:italic>S.\u00a0aureus<\/jats:italic> is able to interact with platelet components resulting in visible changes in PCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Visual inspection of blood components before transfusion is an essential safety practice to interdict the transfusion of bacterially contaminated units.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14049","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T05:26:43Z","timestamp":1487222803000},"page":"1299-1303","source":"Crossref","is-referenced-by-count":27,"title":["Septic transfusion case caused by a platelet pool with visible clotting due to contamination with <i>Staphylococcus aureus<\/i>"],"prefix":"10.1111","volume":"57","author":[{"given":"Maria","family":"Loza\u2010Correa","sequence":"first","affiliation":[{"name":"Canadian Blood Services"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuntong","family":"Kou","sequence":"additional","affiliation":[{"name":"Canadian Blood Services"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariam","family":"Taha","sequence":"additional","affiliation":[{"name":"Canadian Blood Services"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miloslav","family":"Kalab","sequence":"additional","affiliation":[{"name":"Agriculture and Agri\u2010Food Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer","family":"Ronholm","sequence":"additional","affiliation":[{"name":"Health Canada Ottawa Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick M.","family":"Schlievert","sequence":"additional","affiliation":[{"name":"Department of Microbiology Carver College of Medicine Iowa City Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael P.","family":"Cahill","sequence":"additional","affiliation":[{"name":"Department of Microbiology Carver College of Medicine Iowa City Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Skeate","sequence":"additional","affiliation":[{"name":"Department of Microbiology Carver College of Medicine Iowa City Iowa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christine","family":"Cserti\u2010Gazdewich","sequence":"additional","affiliation":[{"name":"University Health Network Toronto Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Ramirez\u2010Arcos","sequence":"additional","affiliation":[{"name":"Canadian Blood Services"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,2,16]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Public Health Agency of Canada. Guideline for Investigation of Suspected Transfusion Transmitted Bacterial Contamination. CCDR2008;34S1:1\u20108 [Internet]. [cited 2017 Jan 20] . Available at:http:\/\/www.phac-aspc.gc.ca\/publicat\/ccdr-rmtc\/08vol34\/34s1\/34s1-eng.php."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2007.01249.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)36592-8"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/529143"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-62703-664-1_10"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1997.15.5.1994"},{"key":"e_1_2_6_8_1","unstructured":"Fatalities reported to FDA following blood collection and transfusion. Annual Summary for Fiscal Year2015[Internet]. Silver Spring (MD): Food and Drug Administration; [cited 2016 Sep 1]. Available from:http:\/\/www.fda.gov\/downloads\/BiologicsBloodVaccines\/SafetyAvailability\/ReportaProblem\/TransfusionDonationFatalities\/UCM518148.pdf."},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-07-655944"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13154"},{"key":"e_1_2_6_11_1","article-title":"One million and counting: bacterial screening of platelet components by NHSBT, is it an effective risk reduction measure? 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