{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T06:56:08Z","timestamp":1770447368873,"version":"3.49.0"},"reference-count":41,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,4,28]],"date-time":"2017-04-28T00:00:00Z","timestamp":1493337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Biomedical Excellence for Safer Transfusion group"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Cryopreserved donor lymphocyte infusion (DLI) products are manufactured and administered to treat relapse after allogeneic hematopoietic stem cell transplantation. Reported clinical responses to DLIs vary broadly, even within the same group of patients. While there is an implicit recognition of the fact that different manufacturing protocols may have specific effects on different cell types, cryopreservation protocols are frequently derived from our experience in the cryopreservation of stem cell products and do not account for the heterogeneous functional nature of DLI T\u2010cell populations. Here, we report the results of a prospective, multicenter trial on the effect of four different cryopreservation solutions that were used to freeze DLIs compared to control DLIs that were refrigerated overnight.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Cryopreserved postthawed and refrigerated specimens were analyzed side by side for their T\u2010cell subpopulation content and viability, as well as T\u2010cell proliferation, cytokine secretion, and cytotoxic activities.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>This study indicates that \u201chomemade\u201d 10% dimethyl sulfoxide (DMSO) results in reduced viability of different CD4+ T\u2010cell populations, including T\u2010helper, T\u2010cytotoxic, and T\u2010regulatory populations, and a decrease in their proliferative and cytotoxic response to immunologically relevant stimuli, while the use of solutions containing 5% DMSO with intracellular\u2010like cryoprotectant stabilizers maintains T\u2010cell function at levels similar to refrigerated control samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study has important implications in determining the best cryoprotectant solution for specific clinical applications in allogeneic immunotherapy.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14112","type":"journal-article","created":{"date-parts":[[2017,4,28]],"date-time":"2017-04-28T08:46:44Z","timestamp":1493369204000},"page":"1555-1565","source":"Crossref","is-referenced-by-count":30,"title":["Clinical methods of cryopreservation for donor lymphocyte infusions vary in their ability to preserve functional T\u2010cell subpopulations"],"prefix":"10.1111","volume":"57","author":[{"given":"D. Nicole","family":"Worsham","sequence":"first","affiliation":[{"name":"Hoxworth Blood Center, University of Cincinnati Cincinnati Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u2010Anna","family":"Reems","sequence":"additional","affiliation":[{"name":"University of Utah School of Medicine Salt Lake City Utah"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zbigniew M.","family":"Szczepiorkowski","sequence":"additional","affiliation":[{"name":"Geisel School of Medicine at Dartmouth Lebanon New Hampshire"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H.","family":"McKenna","sequence":"additional","affiliation":[{"name":"University of Minnesota Medical School Minneapolis Minnesota"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Leemhuis","sequence":"additional","affiliation":[{"name":"Hoxworth Blood Center, University of Cincinnati Cincinnati Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aby J.","family":"Mathew","sequence":"additional","affiliation":[{"name":"BioLife Solutions, Inc. Bothell Washington"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose A.","family":"Cancelas","sequence":"additional","affiliation":[{"name":"Hoxworth Blood Center, University of Cincinnati Cincinnati Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Biomedical Excellence for Safer Transfusion (BEST) Collaborative Group","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,4,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V75.3.555.555"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V86.5.2041.bloodjournal8652041"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1053\/bbmt.1999.v5.pm10465105"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1997.15.2.433"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.3109\/10428194.2012.656635"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1705840"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2217\/imt.13.31"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.11.6053"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/cgt.2015.5"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.52"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1407222"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1215134"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1103849"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12138"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03210.x"},{"key":"e_1_2_8_17_1","first-page":"4705","article-title":"Clinical toxicity of cryopreserved circulating progenitor cells infusion","volume":"18","author":"Zambelli A","year":"1998","journal-title":"Anticancer Res"},{"key":"e_1_2_8_18_1","first-page":"121","article-title":"Cardiovascular changes associated with infusion of hematopoietic cell grafts in oncohematological patients\u2014impact of cryopreservation with dimethylsulfoxide","volume":"31","author":"Horacek JM","year":"2009","journal-title":"Exp Oncol"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2006.05.019"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2003.09.016"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1996.36996420755.x"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39199116897.x"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2004.03336.x"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12428"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2011.03238.x"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/14653240902887242"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(92)90052-U"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1984.247.3.C125"},{"key":"e_1_2_8_29_1","first-page":"105","volume-title":"Marrow transplantation: practical and technical aspects of stem cell reconstitution","author":"Rowley SD.","year":"1992"},{"key":"e_1_2_8_30_1","doi-asserted-by":"crossref","first-page":"2352","DOI":"10.1182\/blood.V95.7.2352","article-title":"Granule exocytosis, and not the fas\/fas ligand system, is the main pathway of cytotoxicity mediated by alloantigen\u2010specific CD4(+) as well as CD8(+) cytotoxic T lymphocytes in humans","volume":"95","author":"Yasukawa M","year":"2000","journal-title":"Blood"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2009.03.002"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.146.9.3242"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2343"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0528.2002.017416.x"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aae0477"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI22053"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-051318"},{"key":"e_1_2_8_38_1","unstructured":"FACT\u2010JACIE International Standards for Hematopoietic Cellular Therapy Product Collection Processing and Administration. 6th ed. 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