{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T03:17:18Z","timestamp":1774322238936,"version":"3.50.1"},"reference-count":6,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,6,26]],"date-time":"2017-06-26T00:00:00Z","timestamp":1498435200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Acquired copy\u2010neutral loss of heterozygosity has been described in myeloid malignant progression with an otherwise normal karyotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>CASE REPORT<\/jats:title><jats:p>A 65\u2010year\u2010old woman with <jats:italic>MPL<\/jats:italic>\u2010mutated essential thrombocythemia and progression to myelofibrosis was noted upon routine pretransplant testing to have mixed field reactivity with anti\u2010D and an historic discrepancy in RhD type. The patient had never received transfusions or transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Gel immunoagglutination revealed group A red blood cells and a mixed\u2010field reaction for the D phenotype, with a predominant D\u2010negative population and a small subset of circulating red blood cells carrying the D antigen. Subsequent genomic microarray single nucleotide polymorphism profiling revealed copy\u2010neutral loss of heterozygosity of chromosome 1 p36.33\u2010p34.2, a known molecular mechanism underlying fibrotic progression of <jats:italic>MPL<\/jats:italic>\u2010mutated essential thrombocythemia. The chromosomal region affected by this copy\u2010neutral loss of heterozygosity encompassed the <jats:italic>RHD<\/jats:italic>, <jats:italic>RHCE<\/jats:italic>, and <jats:italic>MPL<\/jats:italic> genes. We propose a model of chronological molecular events that is supported by <jats:italic>RHD<\/jats:italic> zygosity assays in peripheral lymphoid and myeloid\u2010derived cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Copy\u2010neutral loss of heterozygosity events that lead to clonal selection and myeloid malignant progression may also affect the expression of adjacent unrelated genes, including those encoding for blood group antigens. Detection of mixed\u2010field reactions and investigation of discrepant blood typing results are important for proper transfusion support of these patients and can provide useful surrogate markers of myeloproliferative disease progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14201","type":"journal-article","created":{"date-parts":[[2017,6,27]],"date-time":"2017-06-27T03:43:02Z","timestamp":1498534982000},"page":"2136-2139","source":"Crossref","is-referenced-by-count":3,"title":["Acquired RhD mosaicism identifies fibrotic transformation of thrombopoietin receptor\u2010mutated essential thrombocythemia"],"prefix":"10.1111","volume":"57","author":[{"given":"Celina","family":"Montemayor\u2010Garcia","sequence":"first","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Coward","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maher","family":"Albitar","sequence":"additional","affiliation":[{"name":"Research and Development NeoGenomics Laboratories Irvine California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupa","family":"Udani","sequence":"additional","affiliation":[{"name":"Molecular Diagnostic Laboratory Blood Center of Wisconsin Milwaukee Wisconsin"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prachi","family":"Jain","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleftheria","family":"Koklanaris","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minoo","family":"Battiwalla","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Siob\u00e1n","family":"Keel","sequence":"additional","affiliation":[{"name":"Division of Hematology University of Washington Seattle Washington."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harvey G.","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Transfusion Medicine Clinical Center, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. John","family":"Barrett","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sawa","family":"Ito","sequence":"additional","affiliation":[{"name":"Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,26]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-01-131664"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-04-018879"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-02-486050"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2652"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-068106"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277-013-1938-3"}],"container-title":["Transfusion"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Ftrf.14201","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/trf.14201","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T13:52:43Z","timestamp":1695477163000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/trf.14201"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,26]]},"references-count":6,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/trf.14201"],"URL":"https:\/\/doi.org\/10.1111\/trf.14201","archive":["Portico"],"relation":{},"ISSN":["0041-1132","1537-2995"],"issn-type":[{"value":"0041-1132","type":"print"},{"value":"1537-2995","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,26]]}}}