{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T06:57:25Z","timestamp":1790060245935,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,8,24]],"date-time":"2017-08-24T00:00:00Z","timestamp":1503532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000030","name":"Centers for Disease Control and Prevention","doi-asserted-by":"publisher","award":["DD14\u20101406"],"award-info":[{"award-number":["DD14\u20101406"]}],"id":[{"id":"10.13039\/100000030","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Transfusion"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Red blood cell (RBC) alloimmunization occurs at a high frequency in sickle cell anemia (SCA) despite serologic matching for Rh (C\/c, E\/e) and K antigens. RBC minor antigen genotyping allows for prediction of antigens and <jats:italic>RH<\/jats:italic> variants that may lead to alloimmunization.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>RBC antigen genotyping was performed on chronically transfused pediatric SCA patients, using PreciseType human erythrocyte antigen (HEA), <jats:italic>RHCE<\/jats:italic>, and <jats:italic>RHD<\/jats:italic> BeadChip arrays. All patients received C\/c, E\/e, and K serologically matched units (Category 1); patients with prior RBC antibodies were also matched for Fy<jats:sup>a<\/jats:sup>, Jk<jats:sup>b<\/jats:sup>, and any antibodies (Category 2). The RBC genotypes of all leukoreduced (LR) units transfused over a 12\u2010month period were determined by the prototype HEA\u2010LR BeadChip assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>There were 2320 RBC units transfused to 90 patients in 1135 transfusion episodes. Thirty\u2010five (38.9%) patients had homozygous or compound heterozygous <jats:italic>RH<\/jats:italic> variants. Seven new alloantibodies were detected, with alloantibody incidence of 0.706 in 100 units for Category 2 transfusions and 0.068 in 100 units for Category 1 (p\u2009=\u20090.02). Three patients on Category 2 transfusions formed new anti\u2010Js<jats:sup>a<\/jats:sup> and had a higher rate of exposure to Js<jats:sup>a<\/jats:sup> than those who did not form anti\u2010Js<jats:sup>a<\/jats:sup> (20.4 vs. 8.33 exposures\/100 units, p\u2009=\u20090.02). The most frequent mismatches were S (43.9%), Do<jats:sup>a<\/jats:sup> (43.9%), Fy<jats:sup>a<\/jats:sup> (29.2%), M (28.4%), and Jk<jats:sup>b<\/jats:sup> (28.1%).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Alloimmunization incidence was higher in those with prior RBC antibodies, suggesting that past immunologic responders are at higher risk for future alloimmunization and therefore may benefit from more extensive antigen matching beyond C\/c, E\/e, K, Fy<jats:sup>a<\/jats:sup>, and Jk<jats:sup>b<\/jats:sup>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14282","type":"journal-article","created":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T04:07:46Z","timestamp":1503634066000},"page":"2738-2746","source":"Crossref","is-referenced-by-count":39,"title":["Red blood cell minor antigen mismatches during chronic transfusion therapy for sickle cell anemia"],"prefix":"10.1111","volume":"57","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6082-8385","authenticated-orcid":false,"given":"Marianne E.M.","family":"Yee","sequence":"first","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, Department of Pediatrics, Division of Hematology\/Oncology, Emory University School of Medicine Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cassandra D.","family":"Josephson","sequence":"additional","affiliation":[{"name":"Aflac Cancer and Blood Disorders Center, Children's Healthcare of Atlanta, Department of Pediatrics, Division of Hematology\/Oncology, Emory University School of Medicine Atlanta Georgia"},{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine, Emory University School of Medicine Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne M.","family":"Winkler","sequence":"additional","affiliation":[{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine, Emory University School of Medicine Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Webb","sequence":"additional","affiliation":[{"name":"Center for Cancer and Blood Disorders, Children's National Health System, Departments of Hematology and Laboratory Medicine, George Washington University School of Medicine and Health Sciences Washington DC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi L.C.","family":"Luban","sequence":"additional","affiliation":[{"name":"Center for Cancer and Blood Disorders, Children's National Health System, Departments of Hematology and Laboratory Medicine, George Washington University School of Medicine and Health Sciences Washington DC"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Traci","family":"Leong","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Bioinformatics Emory University, Rollins School of Public Health Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean R.","family":"Stowell","sequence":"additional","affiliation":[{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine, Emory University School of Medicine Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ross M.","family":"Fasano","sequence":"additional","affiliation":[{"name":"Center for Transfusion and Cellular Therapies Department of Pathology and Laboratory Medicine, Emory University School of Medicine Atlanta Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,8,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V76.7.1431.1431"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199006073222301"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.12061"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-03-490623"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2002.00126.x"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.24397"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2010.03045.x"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1987.27187121485.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41091086.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1345.072"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pbc.24530"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12940"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13379"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1988.00380110115024"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1997.37497265338.x"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-03-146415"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12987"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tmrv.2016.05.011"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hoc.2016.01.011"},{"key":"e_1_2_8_21_1","unstructured":"Evidence\u2010based 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