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DENV is considered a high\u2010risk pathogen for blood safety. One of the mitigation strategies to prevent arbovirus TTIs is pathogen inactivation. In this study we demonstrate that the amustaline and glutathione (S\u2010303\/GSH) treatment previously found effective against Zika virus in red blood cells (RBCs) is also effective in inactivating DENV.<\/jats:p><\/jats:sec><jats:sec><jats:title>STUDY DESIGN AND METHODS<\/jats:title><jats:p>Red blood cells were spiked with high levels of DENV. Viral RNA loads and infectious titers were measured in the untreated control and before and after pathogen inactivation treatment of RBC samples. DENV infectivity was also assessed over five successive cell culture passages to detect any potential residual replicative virus.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>The mean\u2009\u00b1\u2009SD DENV titer in RBCs before inactivation was 6.61\u2009\u00b1\u20090.19 log 50% tissue culture infectious dose (TCID<jats:sub>50<\/jats:sub>)\/mL and the mean viral RNA load was 8.42 log genome equivalents\/mL. No replicative DENV was detected either immediately after completion of treatment using S\u2010303\/GSH or after cell culture passages.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>Treatment using S\u2010303\/GSH inactivated high levels of DENV in RBCs to the limit of detection. In combination with previous studies showing the effective inactivation of DENV in plasma and platelets using the licensed amotosalen\/UVA system, this study demonstrates that high levels of DENV can be inactivated in all blood components.<\/jats:p><\/jats:sec>","DOI":"10.1111\/trf.14318","type":"journal-article","created":{"date-parts":[[2017,9,18]],"date-time":"2017-09-18T14:08:36Z","timestamp":1505743716000},"page":"2888-2896","source":"Crossref","is-referenced-by-count":16,"title":["Pathogen inactivation of Dengue virus in red blood cells using amustaline and glutathione"],"prefix":"10.1111","volume":"57","author":[{"given":"Maite","family":"Aubry","sequence":"first","affiliation":[{"name":"P\u00f4le de Recherche et de Veille sur les Maladies Infectieuses \u00c9mergentes, Institut Louis Malard\u00e9 Tahiti, Polyn\u00e9sie Fran\u00e7aise"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew","family":"Laughhunn","sequence":"additional","affiliation":[{"name":"Microbiology Department, Cerus Corporation Concord California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Felicia","family":"Santa Maria","sequence":"additional","affiliation":[{"name":"Microbiology Department, Cerus Corporation Concord California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion C.","family":"Lanteri","sequence":"additional","affiliation":[{"name":"Scientific Affairs Department Cerus Corporation Concord California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adonis","family":"Stassinopoulos","sequence":"additional","affiliation":[{"name":"Scientific Affairs Department Cerus Corporation Concord California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Didier","family":"Musso","sequence":"additional","affiliation":[{"name":"P\u00f4le de Recherche et de Veille sur les Maladies Infectieuses \u00c9mergentes, Institut Louis Malard\u00e9 Tahiti, Polyn\u00e9sie Fran\u00e7aise"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,9,16]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.480"},{"key":"e_1_2_7_3_1","volume-title":"Dengue guidelines for diagnosis, treatment, prevention and control","author":"World Health Organization","year":"2009"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1690"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.2149\/tmh.2011-S05"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004445"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiw122"},{"key":"e_1_2_7_9_1","first-page":"170","article-title":"Review of dengue fever cases in Hong Kong during 1998 to 2005","volume":"14","author":"Chuang V","year":"2008","journal-title":"Hong Kong Med J"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transci.2016.10.013"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiv322"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.12944"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13288"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/trf.13054"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1537-2995.2012.03566.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc0708673"},{"key":"e_1_2_7_17_1","unstructured":"Food and Drug Administration Center for Biologics Evaluation and Research Blood Product Advisory Committee. 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